The transmembrane gradient of the dielectric constant influences the DPH lifetime distribution

• Published in: FEBS letters 1995-11, Vol.374 (3), p.338-340
• Main Authors: Konopásek, Ivo, Kvasnicˇka, Peter, Amler, Evzˇen, Kotyk, Arnosˇt, Curatola, Giovanna
• Format: Article
• Language: English
• Subjects: Cholesterol - pharmacology; Deuterium Oxide - chemistry; Dielectric constant; Diphenylhexatriene - analogs & derivatives; Diphenylhexatriene - chemistry; Electrochemistry; Fluorescence; Fluorescence lifetime distribution; Fluorescent Dyes - chemistry; Heavy water; Lipid-protein interaction; Liposomes - chemistry; Magnetic Resonance Spectroscopy; Phosphatidylcholines - chemistry; Spectrometry, Fluorescence; Water
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)01138-5

The fluorescence lifetime distribution of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-[4-(trimethylamino)phenyll-6-phenyl-1,3,5-hexatriene (TMA-DPH) in egg-phosphatidylcholine liposomes was measured in normal and heavy water. The lower dielectric constant (by ∼12%) of heavy water compared with normal water was employed to provide direct evidence that the drop of the dielectric constant along the membrane normal shifts the centers of the distribution of both DPH and TMA-DPH to higher values and sharpens the widths of the distribution. The profile of the dielectric constant along the membrane normal was not found to be a linear gradient (in contrast to [1]) but a more complex function. Presence of cholesterol in liposomes further shifted the center of the distributions to higher value and sharpened them. In addition, it resulted in a more gradient-like profile of the dielectric constant (i.e. linearization) along the normal of the membrane. The effect of the change of dielectric constant on the membrane proteins is discussed.