Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2)

• Published in: Genomics (San Diego, Calif.) Calif.), 1995-09, Vol.29 (1), p.70-79
• Main Authors: BERMINGHAM, J. R, ARDEN, K. C, NAUMOVA, A. K, SAPIENZA, C, VIARS, C. S, FU, X.-D, KHOTZ, J, MANLEY, J. L, ROSENFELD, M. G
• Format: Article
• Language: English
• Subjects: Alternative Splicing; Animals; Biological and medical sciences; Chromosome Mapping; Chromosomes, Human, Pair 17; Classical genetics, quantitative genetics, hybrids; Cricetinae - genetics; Crosses, Genetic; Female; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Hominidae - genetics; Human; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Male; Mice - genetics; Mice, Inbred Strains - genetics; Nuclear Proteins - genetics; Recombination, Genetic; Ribonucleoproteins; RNA Splicing; RNA-Binding Proteins; Serine-Arginine Splicing Factors; Species Specificity
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1995.1216

The mammalian SR-type splicing factors ASF/SF2 and SC-35 play crucial roles in pre-mRNA splicing and have been shown to shift splice site choice in vitro. We have mapped the ASF/SF2 gene in mice and humans and the SC-35 gene in mice. Somatic cell hybrid mapping of the human ASF/SF2 gene (SFRS1 locus) reveals that it resides on chromosome 17, and fluorescence in situ hybridization refines this localization to 17q21.3-q22. Recombinant inbred mapping of the mouse ASF/SF2 gene (Sfrs1 locus) and the mouse SC-35 gene (Sfrs2 locus) demonstrates that both genes are located in a part of mouse chromosome 11 that is homologous to human chromosome 17. Mapping of Sfrs1 using F1 hybrid backcross mice between the strains C57BL/6 and DDK places Sfrs1 very near the marker D11Mit38 and indicates that the ASF/SF2 gene is closely linked to the Ovum mutant locus.