Hydroxyurea synchronization increases mitotic yield in human glioma cell lines

Karyotypic studies of human gliomas are often limited by a low mitotic index and the appearance of contracted chromosomes that do not exhibit clear banding patterns. The purpose of this study was to investigate the use of hydroxyurea (HU) as a synchronizing agent using established human glioma cell...

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Bibliographic Details
Published in:Acta neuropathologica 1987-01, Vol.73 (3), p.309-312
Main Authors: BIEGEL, J. A, LESLIE, D. S, BIGNER, D. D, BIGNER, S. H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Cycle - drug effects
Cell Line
DNA, Neoplasm - analysis
Flow Cytometry
Glioma - pathology
Humans
Hydroxyurea - pharmacology
Karyotyping - methods
Medical sciences
Mitosis - drug effects
Neurology
Tumors of the nervous system. Phacomatoses
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Summary:Karyotypic studies of human gliomas are often limited by a low mitotic index and the appearance of contracted chromosomes that do not exhibit clear banding patterns. The purpose of this study was to investigate the use of hydroxyurea (HU) as a synchronizing agent using established human glioma cell lines as a model system. HU was shown to reversibly inhibit cellular replication in glioma cell lines U-251MG, D-245MG, D-247MG and D-263MG by flow cytometry on the basis of DNA content. Two-to sixfold increases were demonstrated in the mitotic index of HU-treated cultures exhibiting the greatest percentage of cells in the G2/M phases of the cell cycle. HU, therefore, promises to be an effective agent for use in short term cultures from biopsied human tumors to increase the quality of chromosome preparations in these tumors.
ISSN:0001-6322
1432-0533
DOI:10.1007/BF00686628