Formation of Malondialdehyde-Modified 2′-Deoxyguanosinyl Adduct from Metabolism of Chloral Hydrate by Mouse Liver Microsomes

We previously reported that metabolism of chloral hydrate (CH), a widely used sedative and hypnotic, by male B6C3F1 mouse liver microsomes resulted in lipid peroxidation, producing the tumorigen malondialdehyde (MDA). Now we have found that incubation of CH in the presence of calf thymus DNA resulte...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-11, Vol.216 (3), p.1110-1117
Main Authors: Ni, Y.C., Kadlubar, F.F., Fu, P.P.
Format: Article
Language:English
Subjects:
Animals
Cattle
Chloral Hydrate - metabolism
Chromatography, High Pressure Liquid
Deoxyguanosine - metabolism
DNA - metabolism
DNA Adducts - metabolism
Ethylene Chlorohydrin - analogs & derivatives
Ethylene Chlorohydrin - metabolism
Isotope Labeling
Lipid Peroxidation
Male
Malondialdehyde - metabolism
Malondialdehyde - pharmacology
Mice
Microsomes, Liver - metabolism
Phosphorus Radioisotopes
Trichloroacetic Acid - metabolism
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Summary:We previously reported that metabolism of chloral hydrate (CH), a widely used sedative and hypnotic, by male B6C3F1 mouse liver microsomes resulted in lipid peroxidation, producing the tumorigen malondialdehyde (MDA). Now we have found that incubation of CH in the presence of calf thymus DNA resulted in the formation of an MDA-modified DNA adduct as detected by 32P-postlabeling analysis. Similar results were obtained from incubation of trichloroacetic acid and trichloroethanol, both metabolites of CH.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2735