Gender Identification of Dried Human Bloodstains Using Fluorescence In Situ Hybridization

Identification of the gender of an individual(s) from whom a bloodstain is derived represents important evidence in medicolegal cases. The efficacy of fluorescence in situ hybridization (FISH) using chromosome X and Y centromeric probes was tested to determine its ability to identify correctly the g...

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Bibliographic Details
Published in:Journal of forensic sciences 1995-09, Vol.40 (5), p.885-887
Main Authors: Pettenati, MJ, Rao, PN, Schnell, S, Hayworth-Hodge, R, Lantz, PE, Geisinger, KR
Format: Article
Language:English
Subjects:
Blood Stains
Female
Forensic Medicine - methods
Humans
In Situ Hybridization, Fluorescence
Male
Sex Determination Analysis - methods
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Summary:Identification of the gender of an individual(s) from whom a bloodstain is derived represents important evidence in medicolegal cases. The efficacy of fluorescence in situ hybridization (FISH) using chromosome X and Y centromeric probes was tested to determine its ability to identify correctly the gender of extracted dried bloodstains. In this preliminary study, FISH correctly identified the gender of 2-week-old dried bloodstains in prepared mixtures of male-to-female blood as low as 1%. The technique is accurate, rapid, sensitive, easily performed and readily available. This application of FISH as a forensic laboratory technique holds great promise.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS15403J