Neurotrophin expression modulated by glucocorticoids and oestrogen in immortalized hippocampal neurons

We have used reverse transcription followed by polymerase chain reaction amplification to investigate changes in expression of nerve growth factor (NGF) mRNA in immortalized hippocampal neurons after treatment with the glucocorticoids dexamethasone and corticosterone, the glucocorticoid antagonist R...

Full description

Saved in:
Bibliographic Details
Published in:Brain research. Molecular brain research. 1995-07, Vol.31 (1), p.158-164
Main Authors: Leach Scully, Jackie, Otten, Uwe
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biochemistry and metabolism
Biological and medical sciences
Cell Survival - drug effects
Central nervous system
Corticosterone - antagonists & inhibitors
Corticosterone - pharmacology
Dexamethasone - antagonists & inhibitors
Dexamethasone - pharmacology
Estradiol - pharmacology
Fundamental and applied biological sciences. Psychology
Glucocorticoid
Glucocorticoids - antagonists & inhibitors
Glucocorticoids - pharmacology
Hippocampus
Hippocampus - cytology
Hippocampus - drug effects
Hippocampus - metabolism
Mice
Mifepristone - pharmacology
Molecular Sequence Data
Nerve growth factor
Nerve Growth Factors - genetics
Neuron
Neurons - drug effects
Neurons - metabolism
Neurotrophin-3
Oestrogen
Progesterone - antagonists & inhibitors
Progesterone - pharmacology
RNA, Messenger - biosynthesis
Tumor Cells, Cultured
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have used reverse transcription followed by polymerase chain reaction amplification to investigate changes in expression of nerve growth factor (NGF) mRNA in immortalized hippocampal neurons after treatment with the glucocorticoids dexamethasone and corticosterone, the glucocorticoid antagonist RU38486, and the gonadal steroids progesterone and 17-beta oestradiol. We found that NGF mRNA levels rise after application of either dexamethasone or corticosterone, and that this rise is prevented by the antagonist. Thus, neurotrophin expression is modulated by the physiological glucocorticoid and is mediated by type II glucocorticoid receptors. Progesterone has no effect, while 17-beta oestradiol suppresses NGF mRNA in a postnatally-derived cell line but does not change levels in an embryonic line. An increase in neurotrophin expression is therefore not a general response to steroid hormone application, and may be a specific defence against the presence of metabolically endangering glucocorticoids.
ISSN:0169-328X
1872-6941
DOI:10.1016/0169-328X(95)00047-V