Identification and localization of glycoprotein B expression in lymphoid tissues of chickens infected with turkey herpesvirus

One-day-old chickens were inoculated with turkey herpesvirus (HVT) using an indirect immunofluorescence assay with a monoclonal antibody against HVT glycoprotein B (gB), we determined the course of productive HVT infection in peripheral blood mono-nuclear cells (PBMCs), spleen, thymus, and bursa. PB...

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Bibliographic Details
Published in:Avian diseases 1994-07, Vol.38 (3), p.446-453
Main Authors: Holland, M.S. (Michigan State University, East Lansing, MI.), Silva, R.F, Mackenzie, C.D, Bull, R.W, Witter, R.L
Format: Article
Language:English
Subjects:
Animals
ANTIGENE
ANTIGENOS
Antigens
Antigens, Viral - metabolism
BAZO
BOLSA DE FABRICIO
BOURSE DE FABRICIUS
Bursa of Fabricius - virology
Chickens
Female
Fixatives
Fluorescent Antibody Technique
Gallid herpesvirus 2
GLICOPROTEINAS
GLYCOPROTEINE
Glycoproteins
Herpesviridae
Herpesvirus 2, Gallid - immunology
Herpesvirus 2, Gallid - isolation & purification
Herpesvirus 2, Gallid - metabolism
HERPETOVIRIDAE
Infections
Leukocytes, Mononuclear - virology
Lymphoid Tissue - virology
Male
Marek Disease - virology
Meleagrid herpesvirus 1
MONOCITOS
MONOCYTE
POLLITO
POUSSIN
RATE
SISTEMA LINFATICO
Spleen
Spleen - virology
SYSTEME LYMPHATIQUE
Thymus Gland - virology
Time Factors
Viral diseases
Viral Envelope Proteins - immunology
Viral Envelope Proteins - metabolism
Viruses
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Summary:One-day-old chickens were inoculated with turkey herpesvirus (HVT) using an indirect immunofluorescence assay with a monoclonal antibody against HVT glycoprotein B (gB), we determined the course of productive HVT infection in peripheral blood mono-nuclear cells (PBMCs), spleen, thymus, and bursa. PBMCs were examined from days 4 through 35 postinfection (PI). The spleen, thymus, and bursa were examined from 21 through 70 days PI. Although productive Infection in PBMCs was detected at 4 to 12 days Pl, it ended by 14 days PI. Splenic cells expressed gB at 21, 28, 35, and 70 days Pl, whereas the thymus was positive for gB expression at 21 and 35 days PI. The bursa was never positive for gB expression. At 21, 28, 35, and 70 days PI, plaque formation after co-cultivation of PBMCs with chicken embryo fibroblasts indicated the presence of HVT in infected chickens by co-cultivation assays. On the basis of indirect immunofluorescence assay, gB expression in the spleen and thymus indicates a productive HVT infection in chickens
ISSN:0005-2086
1938-4351
DOI:10.2307/1592064