Regulation of opossum kidney (OK) cell Na/Pi cotransport by Pi deprivation involves mRNA stability

Renal proximal tubular Na-dependent phosphate transport (Na/Pi cotransport) has been studied extensively in the opossum kidney (OK) cell line. Recently, we cloned a complementary deoxyribonucleic acid (cDNA) (NaPi-4) from OK cells encoding an apical NaPi cotransport system. OK cells exposed to a low...

Full description

Saved in:
Bibliographic Details
Published in:Pflügers Archiv 1995-08, Vol.430 (4), p.459-463
Main Authors: Markovich, D, Verri, T, Sorribas, V, Forgo, J, Biber, J, Murer, H
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Carrier Proteins - metabolism
Cell Line
Cell Nucleus - metabolism
Dactinomycin - metabolism
Densitometry
DNA, Complementary - metabolism
Kidney - metabolism
Opossums - metabolism
Phosphates - deficiency
RNA, Messenger - biosynthesis
Sodium-Phosphate Cotransporter Proteins
Symporters
Transcription, Genetic
Up-Regulation - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Renal proximal tubular Na-dependent phosphate transport (Na/Pi cotransport) has been studied extensively in the opossum kidney (OK) cell line. Recently, we cloned a complementary deoxyribonucleic acid (cDNA) (NaPi-4) from OK cells encoding an apical NaPi cotransport system. OK cells exposed to a low-Pi medium, as compared to high-Pi media, responded with an increase in Na/Pi cotransport, which was followed by an increase in NaPi-4 messenger ribonucleic acid (mRNA) abundance; maximal stimulation of Na/Pi cotransport was reached in 2 h, with no further increase for up to 16 h. NAPi-4 mRNA abundance was unaltered for 2 h, then increased to a maximum after 6-16 h in cells treated with low Pi medium. NaPi-4 mRNA decay rate was lowered by low-Pi media when compared to high-Pi media, with no increase in the NaPi-4 mRNA transcription rate. These data suggest that the upregulation of Na/Pi cotransport in OK cells by low-Pi media involves two regulatory mechanisms: an immediate (early) increase (after 2 h) in the expression of Na/Pi cotransport, independent of mRNA synthesis or stability, and a delayed (late) effect (after 4-6 h), resulting in an increase in NaPi-4 mRNA abundance, due to an increased stability.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00373881