The Two Mannose 6-Phosphate Receptors Transport Distinct Complements of Lysosomal Proteins ()

Mammalian cells express two different mannose 6-phosphate receptors (MPR 46 and MPR 300), which both mediate targeting of Man-6-P-containing lysosomal proteins to lysosomes. To assess the contribution of either and both MPRs to the transport of lysosomal proteins, fibroblasts were established from m...

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Bibliographic Details
Published in:The Journal of biological chemistry 1995-11, Vol.270 (45), p.27311-27318
Main Authors: Pohlmann, Regina, Boeker, Martin Wendland Christian, von Figura, Kurt
Format: Article
Language:English
Subjects:
Animals
Biological Transport, Active
Biomarkers
Cathepsin D - metabolism
Cells, Cultured
Fibroblasts - metabolism
Fibroblasts - ultrastructure
Glucuronidase - metabolism
Lysosomes - metabolism
Mice
Microscopy, Immunoelectron
Protein Binding
Proteins - metabolism
Receptor, IGF Type 2 - deficiency
Receptor, IGF Type 2 - genetics
Receptor, IGF Type 2 - metabolism
Solubility
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Summary:Mammalian cells express two different mannose 6-phosphate receptors (MPR 46 and MPR 300), which both mediate targeting of Man-6-P-containing lysosomal proteins to lysosomes. To assess the contribution of either and both MPRs to the transport of lysosomal proteins, fibroblasts were established from mouse embryos that were homozygous for disrupted alleles of either MPR 46 or MPR 300 or both MPRs. Fibroblasts missing both MPRs secreted most of the newly synthesized lysosomal proteins and were unable to maintain the catabolic function of lysosomes. The intracellular levels of lysosomal proteins decreased to
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.270.45.27311