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A comparison between potencies of external calcium, strontium and barium to support GABAergic synaptic transmission in rat cultured hippocampal neurons
Relative potencies of external Ca 2+, Sr 2+ and Ba 2+ to trigger GABAergic synaptic transmission were evaluated by applying the patch-clamp technique to both presynaptic and postsynaptic hippocampal neurons prepared from neonatal rats. Action potentials were evoked by application of voltage pulses t...
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| Published in: | Neuroscience research 1994-09, Vol.20 (3), p.223-229 |
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| cites | cdi_FETCH-LOGICAL-c357t-459ee54b60c6446bcc818d2497d09cd15e9244eb7c4c21a4b1dbe5ca75dd85913 |
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| creator | Ohno-Shosaku, Takako Sawada, Satsuki Hirata, Kazunari Yamamoto, Chosaburo |
| description | Relative potencies of external Ca
2+, Sr
2+ and Ba
2+ to trigger GABAergic synaptic transmission were evaluated by applying the patch-clamp technique to both presynaptic and postsynaptic hippocampal neurons prepared from neonatal rats. Action potentials were evoked by application of voltage pulses to presynaptic neurons, and GABAergic synaptic currents were recorded in voltage-clamped postsynaptic neurons. No stimuli were delivered during replacement with test solutions and only five pulses were applied to the presynaptic neuron in each test solution. During the five-pulse application, the amplitude of synaptic currents was constant in Ca
2+-containing solutions, but decreased successively in Ba
2+- and Sr
2+-containing solutions without Ca
2+. Thus, the amplitude of synaptic currents induced by the first pulse in each ionic condition was used to evaluate the potency of divalent cations. The lowest external concentration required to trigger the transmission was 0.3 mM for Ca
2+, 1 mM for Sr
2+ and 2 mM for Ba
2+, and the concentration required to achieve the same effect as with 2 mM Ca
2+ was 6 mM for Sr
2+ and 10 mM for Ba
2+. These results strongly suggest that Ba
2+ as well as Sr
2+ can be substituted for Ca
2+ in GABAergic synaptic transmission and the order of potency is Ca
2+ > Sr
2+ > Ba
2+. |
| doi_str_mv | 10.1016/0168-0102(94)90091-4 |
| format | article |
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2+, Sr
2+ and Ba
2+ to trigger GABAergic synaptic transmission were evaluated by applying the patch-clamp technique to both presynaptic and postsynaptic hippocampal neurons prepared from neonatal rats. Action potentials were evoked by application of voltage pulses to presynaptic neurons, and GABAergic synaptic currents were recorded in voltage-clamped postsynaptic neurons. No stimuli were delivered during replacement with test solutions and only five pulses were applied to the presynaptic neuron in each test solution. During the five-pulse application, the amplitude of synaptic currents was constant in Ca
2+-containing solutions, but decreased successively in Ba
2+- and Sr
2+-containing solutions without Ca
2+. Thus, the amplitude of synaptic currents induced by the first pulse in each ionic condition was used to evaluate the potency of divalent cations. The lowest external concentration required to trigger the transmission was 0.3 mM for Ca
2+, 1 mM for Sr
2+ and 2 mM for Ba
2+, and the concentration required to achieve the same effect as with 2 mM Ca
2+ was 6 mM for Sr
2+ and 10 mM for Ba
2+. These results strongly suggest that Ba
2+ as well as Sr
2+ can be substituted for Ca
2+ in GABAergic synaptic transmission and the order of potency is Ca
2+ > Sr
2+ > Ba
2+.</description><identifier>ISSN: 0168-0102</identifier><identifier>EISSN: 1872-8111</identifier><identifier>DOI: 10.1016/0168-0102(94)90091-4</identifier><identifier>PMID: 7838423</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Animals, Newborn ; Ba 2 ; Barium - pharmacology ; Ca 2 ; Calcium - pharmacology ; Cations, Divalent - pharmacology ; Cells, Cultured ; Divalent cation ; Evoked Potentials - drug effects ; Evoked Potentials - physiology ; GABAergic synapse ; gamma-Aminobutyric Acid - physiology ; Hippocampal neuron ; Hippocampus - cytology ; Hippocampus - drug effects ; Neurons - drug effects ; Patch-Clamp Techniques ; Potassium Channels - drug effects ; Rats ; Sr 2 ; Strontium - pharmacology ; Synapses - physiology ; Synaptic Transmission - drug effects ; Transmitter release</subject><ispartof>Neuroscience research, 1994-09, Vol.20 (3), p.223-229</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-459ee54b60c6446bcc818d2497d09cd15e9244eb7c4c21a4b1dbe5ca75dd85913</citedby><cites>FETCH-LOGICAL-c357t-459ee54b60c6446bcc818d2497d09cd15e9244eb7c4c21a4b1dbe5ca75dd85913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0168010294900914$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7838423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohno-Shosaku, Takako</creatorcontrib><creatorcontrib>Sawada, Satsuki</creatorcontrib><creatorcontrib>Hirata, Kazunari</creatorcontrib><creatorcontrib>Yamamoto, Chosaburo</creatorcontrib><title>A comparison between potencies of external calcium, strontium and barium to support GABAergic synaptic transmission in rat cultured hippocampal neurons</title><title>Neuroscience research</title><addtitle>Neurosci Res</addtitle><description>Relative potencies of external Ca
2+, Sr
2+ and Ba
2+ to trigger GABAergic synaptic transmission were evaluated by applying the patch-clamp technique to both presynaptic and postsynaptic hippocampal neurons prepared from neonatal rats. Action potentials were evoked by application of voltage pulses to presynaptic neurons, and GABAergic synaptic currents were recorded in voltage-clamped postsynaptic neurons. No stimuli were delivered during replacement with test solutions and only five pulses were applied to the presynaptic neuron in each test solution. During the five-pulse application, the amplitude of synaptic currents was constant in Ca
2+-containing solutions, but decreased successively in Ba
2+- and Sr
2+-containing solutions without Ca
2+. Thus, the amplitude of synaptic currents induced by the first pulse in each ionic condition was used to evaluate the potency of divalent cations. The lowest external concentration required to trigger the transmission was 0.3 mM for Ca
2+, 1 mM for Sr
2+ and 2 mM for Ba
2+, and the concentration required to achieve the same effect as with 2 mM Ca
2+ was 6 mM for Sr
2+ and 10 mM for Ba
2+. These results strongly suggest that Ba
2+ as well as Sr
2+ can be substituted for Ca
2+ in GABAergic synaptic transmission and the order of potency is Ca
2+ > Sr
2+ > Ba
2+.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Ba 2</subject><subject>Barium - pharmacology</subject><subject>Ca 2</subject><subject>Calcium - pharmacology</subject><subject>Cations, Divalent - pharmacology</subject><subject>Cells, Cultured</subject><subject>Divalent cation</subject><subject>Evoked Potentials - drug effects</subject><subject>Evoked Potentials - physiology</subject><subject>GABAergic synapse</subject><subject>gamma-Aminobutyric Acid - physiology</subject><subject>Hippocampal neuron</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - drug effects</subject><subject>Neurons - drug effects</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels - drug effects</subject><subject>Rats</subject><subject>Sr 2</subject><subject>Strontium - pharmacology</subject><subject>Synapses - physiology</subject><subject>Synaptic Transmission - drug effects</subject><subject>Transmitter release</subject><issn>0168-0102</issn><issn>1872-8111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9UcFu1DAQtSpQ2Zb-QZF8Qq1EwE6ctX2ptFRQkCr1AmfLGc-CUWIH26H0S_q7eLurHjlYHmneezPzHiHnnL3njK8_1Kcaxll7ocWlZkzzRhyRFVeybRTn_AVZPUNekZOcfzHGOi26Y3IsVadE263I44ZCnGabfI6BDljuEQOdY8EAHjONW4p_C6ZgRwp2BL9M72guKYZSS2qDo0Ml17JEmpd5jqnQm83HDaYfHmh-CHYutSjJhjz5nH0d4wNNtlBYxrIkdPSnrzywdY2RBlyqeH5NXm7tmPHs8J-S758_fbv-0tze3Xy93tw20PWyNKLXiL0Y1gzWQqwHAMWVa4WWjmlwvEfdCoGDBAEtt2LgbsAerOydU73m3Sl5u9edU_y9YC6mLgk4jjZgXLKRUjKuVV-BYg-EFHNOuDVz8pNND4Yzs8vD7Mw2O7ONFuYpDyMq7c1BfxkmdM-kQwC1f7XvYz3yj8dkcvU9ADqfEIpx0f9_wD9Avp26</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Ohno-Shosaku, Takako</creator><creator>Sawada, Satsuki</creator><creator>Hirata, Kazunari</creator><creator>Yamamoto, Chosaburo</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940901</creationdate><title>A comparison between potencies of external calcium, strontium and barium to support GABAergic synaptic transmission in rat cultured hippocampal neurons</title><author>Ohno-Shosaku, Takako ; Sawada, Satsuki ; Hirata, Kazunari ; Yamamoto, Chosaburo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-459ee54b60c6446bcc818d2497d09cd15e9244eb7c4c21a4b1dbe5ca75dd85913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Ba 2</topic><topic>Barium - pharmacology</topic><topic>Ca 2</topic><topic>Calcium - pharmacology</topic><topic>Cations, Divalent - pharmacology</topic><topic>Cells, Cultured</topic><topic>Divalent cation</topic><topic>Evoked Potentials - drug effects</topic><topic>Evoked Potentials - physiology</topic><topic>GABAergic synapse</topic><topic>gamma-Aminobutyric Acid - physiology</topic><topic>Hippocampal neuron</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - drug effects</topic><topic>Neurons - drug effects</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels - drug effects</topic><topic>Rats</topic><topic>Sr 2</topic><topic>Strontium - pharmacology</topic><topic>Synapses - physiology</topic><topic>Synaptic Transmission - drug effects</topic><topic>Transmitter release</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohno-Shosaku, Takako</creatorcontrib><creatorcontrib>Sawada, Satsuki</creatorcontrib><creatorcontrib>Hirata, Kazunari</creatorcontrib><creatorcontrib>Yamamoto, Chosaburo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohno-Shosaku, Takako</au><au>Sawada, Satsuki</au><au>Hirata, Kazunari</au><au>Yamamoto, Chosaburo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison between potencies of external calcium, strontium and barium to support GABAergic synaptic transmission in rat cultured hippocampal neurons</atitle><jtitle>Neuroscience research</jtitle><addtitle>Neurosci Res</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>20</volume><issue>3</issue><spage>223</spage><epage>229</epage><pages>223-229</pages><issn>0168-0102</issn><eissn>1872-8111</eissn><abstract>Relative potencies of external Ca
2+, Sr
2+ and Ba
2+ to trigger GABAergic synaptic transmission were evaluated by applying the patch-clamp technique to both presynaptic and postsynaptic hippocampal neurons prepared from neonatal rats. Action potentials were evoked by application of voltage pulses to presynaptic neurons, and GABAergic synaptic currents were recorded in voltage-clamped postsynaptic neurons. No stimuli were delivered during replacement with test solutions and only five pulses were applied to the presynaptic neuron in each test solution. During the five-pulse application, the amplitude of synaptic currents was constant in Ca
2+-containing solutions, but decreased successively in Ba
2+- and Sr
2+-containing solutions without Ca
2+. Thus, the amplitude of synaptic currents induced by the first pulse in each ionic condition was used to evaluate the potency of divalent cations. The lowest external concentration required to trigger the transmission was 0.3 mM for Ca
2+, 1 mM for Sr
2+ and 2 mM for Ba
2+, and the concentration required to achieve the same effect as with 2 mM Ca
2+ was 6 mM for Sr
2+ and 10 mM for Ba
2+. These results strongly suggest that Ba
2+ as well as Sr
2+ can be substituted for Ca
2+ in GABAergic synaptic transmission and the order of potency is Ca
2+ > Sr
2+ > Ba
2+.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>7838423</pmid><doi>10.1016/0168-0102(94)90091-4</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-0102 |
| ispartof | Neuroscience research, 1994-09, Vol.20 (3), p.223-229 |
| issn | 0168-0102 1872-8111 |
| language | eng |
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| source | ScienceDirect |
| subjects | Animals Animals, Newborn Ba 2 Barium - pharmacology Ca 2 Calcium - pharmacology Cations, Divalent - pharmacology Cells, Cultured Divalent cation Evoked Potentials - drug effects Evoked Potentials - physiology GABAergic synapse gamma-Aminobutyric Acid - physiology Hippocampal neuron Hippocampus - cytology Hippocampus - drug effects Neurons - drug effects Patch-Clamp Techniques Potassium Channels - drug effects Rats Sr 2 Strontium - pharmacology Synapses - physiology Synaptic Transmission - drug effects Transmitter release |
| title | A comparison between potencies of external calcium, strontium and barium to support GABAergic synaptic transmission in rat cultured hippocampal neurons |
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