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Purification and cDNA Cloning of an Antifungal Protein from the Hemolymph of Holotrichia diomphalia Larvae

An antifungal protein (AFP), holotricin 3, was purified from the hemolymph of the coleopteran insect Holotrichia diomphalia larvae. Analysis of its cDNA showed that holotricin 3 is a novel Gly-and His-rich protein consisting of 84 amino acid residues. This protein was similar to AFP, an antifungal p...

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Published in:	Biological & pharmaceutical bulletin 1995/08/15, Vol.18(8), pp.1049-1052
Main Authors:	LEE, SoYoung, MOON, HyunJoo, KURATA, Shoichiro, NATORI, Shunji, LEE, BokLuel
Format:	Article
Language:	English
Subjects:	Amino Acid Sequence Animals Antifungal Agents - isolation & purification antifungal protein Base Sequence Biological and medical sciences Cloning, Molecular Coleoptera Coleoptera - metabolism DNA, Complementary General pharmacology Gly-, His-rich protein Hemolymph - metabolism insect Insect Hormones - genetics Insect Hormones - isolation & purification Insect Proteins Larva - metabolism Medical sciences Molecular Sequence Data Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Scarabaeidae Sequence Homology, Amino Acid
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cited_by	cdi_FETCH-LOGICAL-c637t-119f80380d58c4d09bb400aa0611afb5db2cc22ec74fa579ad59dbbdcdc8b9973
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container_title	Biological & pharmaceutical bulletin
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creator	LEE, SoYoung MOON, HyunJoo KURATA, Shoichiro NATORI, Shunji LEE, BokLuel
description	An antifungal protein (AFP), holotricin 3, was purified from the hemolymph of the coleopteran insect Holotrichia diomphalia larvae. Analysis of its cDNA showed that holotricin 3 is a novel Gly-and His-rich protein consisting of 84 amino acid residues. This protein was similar to AFP, an antifungal protein of Sarcophaga peregrina that was reported previously, in terms of molecular size and high content of Gly and His residues. However, no appreciable sequence similarity was detected between the two proteins.
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subjects	Amino Acid Sequence Animals Antifungal Agents - isolation & purification antifungal protein Base Sequence Biological and medical sciences Cloning, Molecular Coleoptera Coleoptera - metabolism DNA, Complementary General pharmacology Gly-, His-rich protein Hemolymph - metabolism insect Insect Hormones - genetics Insect Hormones - isolation & purification Insect Proteins Larva - metabolism Medical sciences Molecular Sequence Data Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Scarabaeidae Sequence Homology, Amino Acid
title	Purification and cDNA Cloning of an Antifungal Protein from the Hemolymph of Holotrichia diomphalia Larvae
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