Tissue expression of the Schistosoma mansoni 28 kDa glutathione S-transferase

The expression of the Schistosoma mansoni 28 kDa glutathione S-transferase (Sm28) was studied using molecular (PCR, in situ hybridization), and immunocytochemical techniques. The presence of Sm28 was demonstrated in all developmental stages of the parasite except the intra-uterine immature egg. In t...

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Bibliographic Details
Published in:Parasitology 1994-12, Vol.109 (5), p.565-572
Main Authors: Porchet, E., McNair, A., Caron, A., Kusnierz, J. P., Zemzoumi, K., Capron, A.
Format: Article
Language:English
Subjects:
Animals
Antigens, Helminth
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Biomphalaria
Cricetinae
developmental stages
DNA Primers - genetics
DNA, Helminth - genetics
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
glutathione S-transferase
Glutathione Transferase - genetics
Glutathione Transferase - immunology
Glutathione Transferase - metabolism
Immunohistochemistry
In Situ Hybridization
Invertebrates
Male
Mesocricetus
Molecular Sequence Data
Nemathelminthia. Plathelmintha
Physiology. Development
Polymerase Chain Reaction
Schistosoma mansoni
Schistosoma mansoni - enzymology
Schistosoma mansoni - genetics
Schistosoma mansoni - growth & development
Tissue Distribution
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Summary:The expression of the Schistosoma mansoni 28 kDa glutathione S-transferase (Sm28) was studied using molecular (PCR, in situ hybridization), and immunocytochemical techniques. The presence of Sm28 was demonstrated in all developmental stages of the parasite except the intra-uterine immature egg. In the parenchyma of male and female adult worms the distribution of Sm28 was limited to a subpopulation of parenchymal cells and to the dorsal tubercles of the male. The tegument, the muscles, the digestive tract, the neural mass, the vitelline glands, and mature gametes were not immunoreactive. Immature germinal cells in both sexes, and the ootype in the female genital system, were found to express Sm28. Deposits of immunoreactive material on host skin following cercarial penetration, exfoliation from the male tubercles, and especially emission of Sm28 from eggs in hepatic granulomas are suspected to be a source of antigen during the parasite infection. The reduction in worm fecundity previously observed in immunization experiments may result from an antibody response directed against Sm28 present in the ootype. There was no cross-reactivity observed, under the experimental conditions used, between the anti-Sm28 sera and either vertebrate or invertebrate host tissue.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000076447