Preliminary data from investigations of the in vivo biopersistence of three experimental glass fibres of varying chemical composition

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 1995, Vol.47 (2), p.179-182
• Main Authors: Morris, K.J., Launder, K.A., Morgan, A., Hornby, S.B., Collier, C.G.
• Format: Article
• Language: English
• Subjects: Animals; Asbestosis; Biological and medical sciences; Biopersistence, glass fibres; Chemical and industrial products toxicology. Toxic occupational diseases; Female; Glass - chemistry; Glass fibres, experimental; Inorganic dusts (pneumoconiosises) and organic dusts (byssinosis etc.); Intubation, Intratracheal; Lung - chemistry; Lung - pathology; Medical sciences; Mucociliary Clearance; Rats; Rats, Inbred F344; Solubility; Time Factors; Toxicology
• ISSN: 0940-2993; 1618-1433
• DOI: 10.1016/S0940-2993(11)80311-8

The durability of three experimental glass fibres (X7753, X7484, and X7779) was investigated in vivo. These fibres had in vitro dissolution rates of 600, 150, and 2 ng cm -2 hour -1, respectively. Three groups of female Fischer-344 rats were intratracheally instilled with a 1.2 mg suspension of one of each of the fibre types. All fibres had previously been neutron activated, to produce radioactive 24Na within the glass, which served as a radiotracer. At 2 days post instillation (PI) about 1 × 10 6 glass fibres were within the pulmonary region of the lung. Animals were killed at various time points from 2 to 360 days PI. Fibres were recovered from the animal lungs by hypochlorite digestion. The retention and morphometry of these fibres was investigated, and preliminary results are presented. After 360 days in the lung, the number of X7753 and X7484 fibres fell respectively to 10 % and 50 % of those present at 2 days PL There was no detectable reduction in the number of X7779 fibres in the lung over this period. Morphometric analyses demonstrated a 53 % and 22 % reduction in the mean length of the X7753 and X7484 fibres, after 360 days in the lung. Reduction in diameter was apparent after only 28 days for the these fibre types. No change in the mean size of the X7779 fibres was observed during the study. The fibre morphometry data suggested that short fibres dissolved at a slower rate than long fibres. In general the in vivo fibre retention and morphometry data reflected the measured in vitro dissolution rate.