Biochemical and spectroscopic characterization of the putative photoreceptor for phototaxis in amoebae of the cellular slime mould Dictyostelium discoideum

Using sucrose gradient centrifugation, anion exchange chromatography on a SMART system, isoelectric focusing (IEF) and sodium dodecylsulphate polycrylamide gel electrophoresis (SDS PAGE), a 45.5. kDa membrane protein was isolated from amoebae of the cellular slime mould Dictyostelium discoideum. The...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 1995-10, Vol.30 (2), p.139-143
Main Authors: Schlenkrich, Thomas, Fleischmann, Peter, Häder, Donat-P.
Format: Article
Language:English
Subjects:
Action spectrum
Animals
Cell Movement
Dictyostelium - chemistry
Dictyostelium - physiology
Dictyostelium discoideum
Dictyostelium discoideum amoebae
Light
Membrane Proteins - chemistry
Membrane Proteins - physiology
Photoreceptor
Photoreceptor Cells, Invertebrate - physiology
Phototaxis
Spectrophotometry
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Summary:Using sucrose gradient centrifugation, anion exchange chromatography on a SMART system, isoelectric focusing (IEF) and sodium dodecylsulphate polycrylamide gel electrophoresis (SDS PAGE), a 45.5. kDa membrane protein was isolated from amoebae of the cellular slime mould Dictyostelium discoideum. The absorption spectrum of the isolated protein corresponds well with the action spectrum for the photoaccumulation of the amoebae of Dictyostelium discoideum showing a major peak between 405 and 412 nm and some minor peaks between 500 and 600 nm. The remarkable similarity of the two spectra leads to the hypothesis that the isolated protein-pigment complex may serve as a photoreceptor for amoebal phototaxis.
ISSN:1011-1344
1873-2682
DOI:10.1016/1011-1344(95)07184-4