Molecular cloning of the human gene SUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation

DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this seconda...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1995-07, Vol.21 (4), p.255-263
Main Authors: Rosenstein, B S, Vaslet, C A
Format: Article
Language:English
Subjects:
Adult
Animals
Bromodeoxyuridine
Cell Line
Cell Survival - radiation effects
Cloning, Molecular
Cross-Linking Reagents
DNA - metabolism
DNA - radiation effects
DNA Repair
Female
Fibroblasts - cytology
Fibroblasts - metabolism
Fibroblasts - radiation effects
Humans
Kinetics
Light
Photolysis
Proteins - metabolism
Proteins - radiation effects
Ranidae
Skin - cytology
Skin - metabolism
Skin - radiation effects
Sunlight
Time Factors
Transfection
Ultraviolet Rays
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Description
Summary:DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring a normal DPC response and solar UV sensitivity to DRP 153. This gene has been designated SUVCC3 to denote solar UV cross-complementing gene number 3.
ISSN:0740-7750
DOI:10.1007/BF02255780