Characterization of squid enolase mRNA : sequence analysis, tissue distribution, and axonal localization

Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). Howev...

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Bibliographic Details
Published in:Neurochemical research 1995-08, Vol.20 (8), p.923-930
Main Authors: CHUN, J. T, GIOIO, A. E, CRISPINO, M, GIUDITTA, A, KAPLAN, B. B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Axons - metabolism
Base Sequence
Biochemistry. Physiology. Immunology
Biological and medical sciences
Cloning, Molecular
Decapodiformes
DNA, Complementary - genetics
Fundamental and applied biological sciences. Psychology
Genetic Code
Humans
Invertebrates
Isoenzymes - genetics
Loligo pealei
Marine
Molecular Sequence Data
Mollusca
Organ Specificity - physiology
Phosphopyruvate Hydratase - genetics
Physiology. Development
RNA, Messenger - analysis
Sequence Homology, Amino Acid
Species Specificity
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Summary:Enolase is a glycolytic enzyme whose amino acid sequence is highly conserved across a wide range of animal species. In mammals, enolase is known to be a dimeric protein composed of distinct but closely related subunits: alpha (non-neuronal), beta (muscle-specific), and gamma (neuron-specific). However, little information is available on the primary sequence of enolase in invertebrates. Here we report the isolation of two overlapping cDNA clones and the putative primary structure of the enzyme from the squid (Loligo pealii) nervous system. The composite sequence of those cDNA clones is 1575 bp and contains the entire coding region (1302 bp), as well as 66 and 207 bp of 5' and 3' untranslated sequence, respectively. Cross-species comparison of enolase primary structure reveals that squid enolase shares over 70% sequence identity to vertebrate forms of the enzyme. The greatest degree of sequence similarity was manifest to the alpha isoform of the human homologue. Results of Northern analysis revealed a single 1.6 kb mRNA species, the relative abundance of which differs approximately 10-fold between various tissues. Interestingly, evidence derived from in situ hybridization and polymerase chain reaction experiments indicate that the mRNA encoding enolase is present in the squid giant axon.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00970738