Combinatorial signaling by Sonic hedgehog and Wnt family members induces myogenic bHLH gene expression in the somite

We have demonstrated previously that a combination of signals from the neural tube and the floor plate/notochord complex synergistically induce the expression of myogenic bHLH genes and myogenic differentiation markers in unspecified somites. In this study we demonstrate that Sonic hedgehog (Shh), w...

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Bibliographic Details
Published in:Genes & development 1995-12, Vol.9 (23), p.2911-2922
Main Authors: Münsterberg, A E, Kitajewski, J, Bumcrot, D A, McMahon, A P, Lassar, A B
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Basic Helix-Loop-Helix Transcription Factors
Chick Embryo
DNA-Binding Proteins - genetics
Embryonic and Fetal Development - drug effects
Embryonic Induction
Gene Expression Regulation, Developmental
Hedgehog Proteins
In Vitro Techniques
Mesoderm - drug effects
Mesoderm - physiology
Molecular Sequence Data
Muscles - embryology
MyoD Protein - biosynthesis
MyoD Protein - genetics
Nervous System - embryology
Nervous System - metabolism
Notochord - drug effects
Notochord - embryology
Notochord - metabolism
Paired Box Transcription Factors
Protein Biosynthesis
Proteins - genetics
Proteins - pharmacology
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins - genetics
Signal Transduction
Trans-Activators
Transcription Factors - genetics
Wnt Proteins
Wnt1 Protein
Wnt3 Protein
Wnt4 Protein
Zebrafish Proteins
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Summary:We have demonstrated previously that a combination of signals from the neural tube and the floor plate/notochord complex synergistically induce the expression of myogenic bHLH genes and myogenic differentiation markers in unspecified somites. In this study we demonstrate that Sonic hedgehog (Shh), which is expressed in the floor plate/notochord, and a subset of Wnt family members (Wnt-1, Wnt-3, and Wnt-4), which are expressed in dorsal regions of the neural tube, mimic the muscle inducing activity of these tissues. In combination, Shh and either Wnt-1 or Wnt-3 are sufficient to induce myogenesis in somitic tissue in vitro. Therefore, we propose that myotome formation in vivo may be directed by the combinatorial activity of Shh secreted by ventral midline tissues (floor plate and notochord) and Wnt ligands secreted by the dorsal neural tube.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.9.23.2911