Charting of Jun family member proteins in the rat forebrain and midbrain: immunocytochemical evidence for a new Jun-related antigen

• Published in: Brain research 1995-09, Vol.692 (1), p.1-22
• Main Authors: Harlan, Richard E., Garcia, Meredith M.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies - analysis; Biochemistry and metabolism; Biological and medical sciences; Blotting, Western; Central nervous system; Fundamental and applied biological sciences. Psychology; Genes, jun; Immediate-Early Proteins - analysis; Immediate-Early Proteins - genetics; Immediate-Early Proteins - immunology; Immunoblotting; Immunohistochemistry; In Situ Hybridization; Male; Mesencephalon - anatomy & histology; Mesencephalon - metabolism; Molecular Weight; Prosencephalon - anatomy & histology; Prosencephalon - metabolism; Rats; Rats, Sprague-Dawley; RNA, Messenger - biosynthesis; Vertebrates: nervous system and sense organs
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/0006-8993(95)00624-Y

Immunocytochemistry was used to localize members of the Jun family of immediate-early genes in the forebrain and midbrain of non-stimulated male rats. Antibodies against specific peptide sequences of c-Jun (Ab-1 and Ab-2 from Oncogene Science) and against expressed proteins of JunB and JunD (both from Dr. R. Bravo) revealed widespread and unique distributions for each of these antigens. Charts were made of the distribution of each antigen, and extensive comparisons were made of previous results obtained using in situ hybridization to localize mRNAs for c- jun, junB and junD. Our results indicate a generally favorable comparison between immunoreactivity and distribution of mRNAs for JunB and JunD, but in the case of c-Jun, immunoreactivity and mRNA were comparable only with the Ab-1 antibody. Indeed, the immunocytochemical distribution of the antigen recognized by the c-Jun Ab-2 antibody was distinctly different from that of the other Jun proteins or mRNAs in the rat brain. This antibody (Ab-2) recognized a nuclear protein found extensively in the caudate-putamen, nucleus accumbens, layer II of the olfactory tubercle, the central nucleus of the amygdala, and the lateral division of the bed nucleus of the stria terminalis. Scattered labeled nuclei were found in a few other forebrain structures. Within the caudate-putamen, immunoreactivity was restricted to the matrix compartment, as determined by immunostaining of adjacent sections with the matrix-marker calbindin D 28k. Western blots of caudate-putamen demonstrated that this antibody recognized a protein doublet of molecular masses approximately 37 and 34 kDa, distinct from the molecular masses of c-Jun, JunB and JunD. This unique neuroanatomical distribution and molecular mass suggests that this antibody recognizes a previously undescribed Jun-related antigen.