Functional expression of the renal organic cation transporter and P-glycoprotein in Xenopus laevis oocytes

The hypothesis that P-glycoprotein (P-gp) mediates the renal secretion of organic cations was tested by functional expression of mRNAs in the Xenopus laevis oocyte system. Efflux of 2'-deoxytubercidin (dTub), a substrate for the renal organic cation transporter (OCT) but not for P-gp, was enhan...

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Bibliographic Details
Published in:Cancer chemotherapy and pharmacology 1995-01, Vol.37 (1-2), p.187-189
Main Authors: NELSON, J. A, DUTT, A, ALLEN, L. H, WRIGHT, D. A
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis
Biological and medical sciences
Biological Transport
Carrier Proteins - biosynthesis
Cations - metabolism
Female
Fundamental and applied biological sciences. Psychology
Glycoproteins
Humans
Ion Transport
Kidney - metabolism
Mice
Oocytes - metabolism
Proteins
RNA, Messenger - analysis
Swine
Tubercidin - analogs & derivatives
Tubercidin - metabolism
Xenopus laevis
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Summary:The hypothesis that P-glycoprotein (P-gp) mediates the renal secretion of organic cations was tested by functional expression of mRNAs in the Xenopus laevis oocyte system. Efflux of 2'-deoxytubercidin (dTub), a substrate for the renal organic cation transporter (OCT) but not for P-gp, was enhanced by injection of renal mRNA but not by injection of mRNA from P-gp-overexpressing cells (MDCK cells transduced with the cDNA for human MDR1). The functional capacity of the MDCK-MDR mRNA was established by its ability to reduce the steady-state uptake of a classical P-gp substrate, vinblastine. Thus, these data indicate OCT and P-gp to be distinct entities. The Xenopus oocyte system provides a functional approach to further characterize the OCT.
ISSN:0344-5704
1432-0843
DOI:10.1007/BF00685648