Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector

Hygromycin-resistance (Hy R)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZα (encoding the α-polypeptide fragment of β-galactosidase (βGal))-multiple cloning site cassette into a Hy R-base...

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Bibliographic Details
Published in:Gene 1995-12, Vol.166 (1), p.181-182
Main Authors: Howard, Nathan S., Gomez, James E., Ko, Chiew, Bishai, William R.
Format: Article
Language:English
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Summary:Hygromycin-resistance (Hy R)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZα (encoding the α-polypeptide fragment of β-galactosidase (βGal))-multiple cloning site cassette into a Hy R-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of βGal color selection in appropriate E. coli host strains.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00597-X