Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector
Hygromycin-resistance (Hy R)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZα (encoding the α-polypeptide fragment of β-galactosidase (βGal))-multiple cloning site cassette into a Hy R-base...
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Published in: | Gene 1995-12, Vol.166 (1), p.181-182 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Hygromycin-resistance (Hy
R)-based
Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a
lacZα (encoding the α-polypeptide fragment of β-galactosidase (βGal))-multiple cloning site cassette into a Hy
R-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of βGal color selection in appropriate
E. coli host strains. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(95)00597-X |