Glutamate enhances the adenylyl cyclase-cAMP system-induced beta-endorphin secretion and POMC mRNA expression in rat hypothalamic neurons in culture: NMDA receptor-mediated modulation

l-Glutamate, a major excitatory amino acid of the central nervous system, plays important roles as neurotransmitter and neuromodulator in the brain. Increasing evidence suggests that glutamate may also involve in the regulation of the neuroendocrine system at the hypothalamus. Employing long term mo...

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Bibliographic Details
Published in:Brain research 1995-09, Vol.692 (1), p.129-136
Main Authors: Yang, Zhiyu, Copolov, David L., Lim, Alan T.
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - physiology
Animals
beta-Endorphin - metabolism
Biological and medical sciences
Blotting, Northern
cAMP
Cell culture
Cells, Cultured
Colforsin - pharmacology
Cyclic AMP - physiology
Excitatory Amino Acid Agonists - pharmacology
Fundamental and applied biological sciences. Psychology
Glutamate
Glutamic Acid - pharmacology
Hormones and neuropeptides. Regulation
Hypothalamus
Hypothalamus - cytology
Hypothalamus - drug effects
Hypothalamus - metabolism
Hypothalamus. Hypophysis. Epiphysis. Urophysis
Neurons - drug effects
Neurons - metabolism
NMDA
Pro-Opiomelanocortin - biosynthesis
Radioimmunoassay
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate - drug effects
RNA, Messenger - biosynthesis
Stimulation, Chemical
Vertebrates: endocrinology
β-Endorphin
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Summary:l-Glutamate, a major excitatory amino acid of the central nervous system, plays important roles as neurotransmitter and neuromodulator in the brain. Increasing evidence suggests that glutamate may also involve in the regulation of the neuroendocrine system at the hypothalamus. Employing long term monolayer hypothalamic cell cultures prepared from neonatal rats, we reported here that whereas glutamate significantly enhanced forskolin-, or N 6,2′-O-dibutyrrladenosine-3′5′-cyclic monophosphate [(Bu) 2cAMP]-stimulated immunoreactive (ir)-β EP release form cultures treated daily for 4 consecutive days, the excitatory amino acid alone produced little effect. This potentiation of glutamate was time-related and dose-dependent with an E max value of the amino acid being approximately 50 μM; at this concentration glutamate augmented ir-β EP secretion about 1.8 times ( P
ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(95)00684-I