Proliferation enhancement by spontaneous multiplication of chromosome 7 in rheumatic synovial cells in vitro

Mosaic trisomy of chromosome 7 is known to occur in a variety of non-neoplastic hyperproliferative disorders. In long-term cell cultures established from rheumatic synovium with mosaic trisomy 7, we observed a continuous increase in the proportion of cells with trisomy 7 to over 50% by the 10th in v...

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Bibliographic Details
Published in:Human genetics 1995-12, Vol.96 (6), p.651-654
Main Authors: ERMIS, A, HENN, W, REMBERGER, K, HOPF, C, HOPF, T, ZANG, K. D
Format: Article
Language:English
Subjects:
Arthritis, Rheumatoid - genetics
Arthritis, Rheumatoid - pathology
Biological and medical sciences
Cells, Cultured
Chromosomes, Human, Pair 7
Diseases of the osteoarticular system
DNA Probes
Humans
In Situ Hybridization, Fluorescence
Inflammatory joint diseases
Ki-67 Antigen
Medical sciences
Mosaicism
Neoplasm Proteins - analysis
Nuclear Proteins - analysis
Synovial Membrane - pathology
Trisomy
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Summary:Mosaic trisomy of chromosome 7 is known to occur in a variety of non-neoplastic hyperproliferative disorders. In long-term cell cultures established from rheumatic synovium with mosaic trisomy 7, we observed a continuous increase in the proportion of cells with trisomy 7 to over 50% by the 10th in vitro passage. Simultaneous in situ hybridization with a repetitive chromosome-7-specific DNA probe and fluorescent Ki-67 labelling showed a strong correlation between trisomy 7 and an elevated proliferation index in cultured rheumatic synovial cells. Moreover, we observed a fraction of rapidly proliferating cells with up to eight copies of chromosome 7 as the sole cytogenetic change. Frequent somatic pairing of centromeres of two chromosomes 7 in interphase nuclei suggests either atypical non-disjunction with a persisting centromere or selective endoreduplication of chromosome 7.
ISSN:0340-6717
1432-1203
DOI:10.1007/bf00210293