Alterations to the en bloc basic fuchsin staining protocol for the demonstration of microdamage produced in vivo

En bloc staining with basic fuchsin has become the method of choice to demonstrate bone microdamage produced in vivo. Several alterations have recently been made to the protocol. This technical note presents the new protocols, which include staining through a graded series of alcohols under vacuum,...

Full description

Saved in:
Bibliographic Details
Published in:Bone (New York, N.Y.) N.Y.), 1995-10, Vol.17 (4), p.431-433
Main Authors: Burr, D.B., Hooser, M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone
Bone and Bones
Diseases of the osteoarticular system
Dogs
Ethanol - chemistry
Femur
Histomorphometry
Hydrogen-Ion Concentration
Medical sciences
Methylmethacrylate
Methylmethacrylates - chemistry
Microdamage
Osteoporosis. Osteomalacia. Paget disease
Rosaniline Dyes
Staining and Labeling
Tissue Embedding
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:En bloc staining with basic fuchsin has become the method of choice to demonstrate bone microdamage produced in vivo. Several alterations have recently been made to the protocol. This technical note presents the new protocols, which include staining through a graded series of alcohols under vacuum, and eliminating the original evaporation stage in the staining protocol. Reasons for variations in staining quality can be related to inadequate dehydration, failure to stain under vacuum, the source of the basic fuchsin, or the solubility of basic fuchsin in water. The most common reasons for over- or understaining are the time left in stain, and the density of the bone. Limitations to the technique include the fact that probably not all cracks are stained by the technique, and the technique is not useful for studies that involve bone with highly variable densities in a single section.
ISSN:8756-3282
1873-2763
DOI:10.1016/S8756-3282(95)00241-3