Box I and II motif from myelin basic protein gene promoter binds to nuclear proteins from rodent brain

The box I and II motif located within the promoter of the myelin basic protein gene contains a simian virus 40 T-antigen-binding site, a MyoD/E2a-binding site, and a glucocorticoid receptor-binding site. We have found proteins within nuclear extracts from adult mouse brain, rat embryonic cerebral co...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular neuroscience 1994-03, Vol.5 (1), p.27-37
Main Authors: Kimbro, K S, Rosenberg, P A, Saavedra, R A
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Brain - metabolism
Cell Line
DNA - metabolism
DNA-Binding Proteins - metabolism
Female
Gene Expression Regulation - drug effects
Glucocorticoids - pharmacology
Mice
Molecular Sequence Data
Myelin Basic Protein - genetics
Nuclear Proteins - metabolism
Promoter Regions, Genetic
Rats
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The box I and II motif located within the promoter of the myelin basic protein gene contains a simian virus 40 T-antigen-binding site, a MyoD/E2a-binding site, and a glucocorticoid receptor-binding site. We have found proteins within nuclear extracts from adult mouse brain, rat embryonic cerebral cortex in culture, and a mouse oligodendrocyte-like cell line that bind to a 32P-labeled synthetic DNA fragment containing the sequences of the box I and II motif. Three major complexes (A, B, and C) were seen in gel-shift assays. Only complexes A and B were competed out by the unlabeled box I and II fragment or by another synthetic DNA fragment that also contains sequences similar to a glucocorticoid receptor-binding site. Therefore, complexes A and B were thought to be specific. The expression pattern of the proteins responsible for the formation of these complexes was also assessed during development in mouse brain.
ISSN:0895-8696
1559-1166
DOI:10.1007/BF02736692