A new monoclonal antibody (3D3) generated with human respiratory mucins and directed against Lewis determinants

We have prepared a monoclonal antibody (MAb), 3D3, raised against purified human respiratory mucins. This antibody recognized mucins and proteolytically derived glycopeptides. The epitope recognized by the antibody was destroyed by α-L-fucosidase, indicating that it was present on the carbohydrate m...

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Bibliographic Details
Published in:Glycobiology (Oxford) 1995-09, Vol.5 (6), p.563-570
Main Authors: Nathalie, Emery, Palfaï, Shirley B., Place, Graham, Oriol, Rafael, Hall, Roderick L., Roussel, Philippe, Lhermitte, Michel
Format: Article
Language:English
Subjects:
Adsorption
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Binding Sites, Antibody
Blotting, Western
Bronchi - immunology
Bronchi - metabolism
Carbohydrate Sequence
Enzyme-Linked Immunosorbent Assay
Glycosylation
Hemagglutination Tests
human bronchial mucins
Humans
Immunohistochemistry
Lewis b
Lewis Blood-Group System - immunology
Molecular Sequence Data
Mucins - immunology
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Description
Summary:We have prepared a monoclonal antibody (MAb), 3D3, raised against purified human respiratory mucins. This antibody recognized mucins and proteolytically derived glycopeptides. The epitope recognized by the antibody was destroyed by α-L-fucosidase, indicating that it was present on the carbohydrate moieties. Structural specificity was determined by adsorption on a variety of synthetic, insolubilized oligosaccharides. Several lines of evidence indicate that the 3D3 MAb reacted strongly with the Lewis (Leb) antigen, but also recognized Lea and Ley determinants. This antibody might be useful to study mucin secretion.
ISSN:0959-6658
1460-2423
DOI:10.1093/glycob/5.6.563