Application of a membrane fusion assay for rapid drug screening

The purpose of this study is to develop an in vitro assay for screening drug and their effects on membrane fusion and lysis of intracellular organelles. A 96-well microtiter-dish turbidimetric assay using membrane components of the eggs of sea urchins, a marine invertebrate, was applied to monitor g...

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Bibliographic Details
Published in:Pharmaceutical research 1995-10, Vol.12 (10), p.1417-1422
Main Authors: VOGEL, S. S, BEUSHAUSEN, S, LESTER, D. S
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - metabolism
Drug Evaluation, Preclinical - methods
Drug toxicity and drugs side effects treatment
Exocytosis
Medical sciences
Membrane Fusion
Miscellaneous (drug allergy, mutagens, teratogens...)
Pharmacology. Drug treatments
Sea Urchins
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Summary:The purpose of this study is to develop an in vitro assay for screening drug and their effects on membrane fusion and lysis of intracellular organelles. A 96-well microtiter-dish turbidimetric assay using membrane components of the eggs of sea urchins, a marine invertebrate, was applied to monitor granule fusion and/or lysis. Of 18 drugs screened, 16 had no effect. One antineoplastic drug, tamoxifen, disrupted intracellular membranes in a calcium independent manner. Taxol, another antineoplastic drug, specifically inhibited calcium triggered exocytosis. This assay is inexpensive, simple, rapid, and does not require the sacrifice of animal life. It has the potential to identify drugs that are membrane active, as well as those which specifically perturb events involved in the secretion process.
ISSN:0724-8741
1573-904X
DOI:10.1023/A:1016258615076