Production and purification of hybrid ty-VLPs

This article describes how pure Ty-VLPs (virus-like particles) can be prepared from hybrid Ty-VLPs. Many different hybrid Ty-VLPs have been produced and may be easily purified. Since the sedimentation properties of different hybrid Ty-VLPs are similar, a simple purification process can be used for a...

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Bibliographic Details
Published in:Molecular biotechnology 1994-04, Vol.1 (2), p.137-145, Article 137
Main Authors: Burns, N.R. (British Bio-Technology Ltd., Oxford, UK.), Gilmour, J.E.M, Kingsman, S.M, Kingsman, A.J, Adams, S.E
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic Engineering
Genetic Techniques
Genetic Vectors - biosynthesis
Genetic Vectors - isolation & purification
HIBRIDOS
HYBRIDE
Hybridization, Genetic
HYBRIDS
Methods. Procedures. Technologies
Protein engineering
PROTEINAS
PROTEINE
PROTEINS
PURIFICACION
PURIFICATION
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Retroelements
RETROTRANSPOSONS
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Viral Proteins - biosynthesis
Viral Proteins - genetics
VIRUS
VIRUS-LIKE PARTICLES
VIRUSES
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Summary:This article describes how pure Ty-VLPs (virus-like particles) can be prepared from hybrid Ty-VLPs. Many different hybrid Ty-VLPs have been produced and may be easily purified. Since the sedimentation properties of different hybrid Ty-VLPs are similar, a simple purification process can be used for any VLP. This fast, versatile, and easy process allows for the production of a variety of recombinant proteins.
ISSN:1073-6085
1559-0305
DOI:10.1007/BF02921554