Regulation of 60-kDa heat shock protein expression by systemic stress and 5-hydroxytryptamine in rat colonic mucosa

Bowel dysfunction such as irritable bowel syndrome caused by stress is well described. Previous reports suggest that 5-hydroxytryptamine (5-HT) mediates alteration of bowel motility. In this study, the effects of water-immersion stress and the administration of 5-HT on the expression of a 60-kDa hea...

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Bibliographic Details
Published in:Journal of gastroenterology 1994-12, Vol.29 (6), p.721-726
Main Authors: Kuwabara, T, Otaka, M, Itoh, H, Zeniya, A, Fujimori, S, Otani, S, Tashima, Y, Masamune, O
Format: Article
Language:English
Subjects:
Animals
Benzimidazoles - pharmacology
Chaperonin 60 - metabolism
Colon - drug effects
Colon - metabolism
Gastrointestinal Motility - drug effects
Immersion
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Male
Rats
Rats, Sprague-Dawley
Serotonin - pharmacology
Serotonin Antagonists - pharmacology
Stress, Physiological - metabolism
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Summary:Bowel dysfunction such as irritable bowel syndrome caused by stress is well described. Previous reports suggest that 5-hydroxytryptamine (5-HT) mediates alteration of bowel motility. In this study, the effects of water-immersion stress and the administration of 5-HT on the expression of a 60-kDa heat shock protein (HSP60) in rat colonic mucosa were investigated. The effect of YM-060, a 5-hydroxytryptamine 3 (5-HT3) receptor antagonist, on the expression of this protein was also studied. Water-immersion stress and the administration of 5-HT induced synthesis of HSP60 in rat colonic mucosa. The induction of HSP60 and the number of defecations were clearly inhibited by the oral administration of YM-060. Our results suggest that the induction of HSP60 in rat colonic mucosa by water-immersion stress may be associated with gastrointestinal motility mediated by 5-HT, especially via 5-HT3 receptors.
ISSN:0944-1174
1435-5922
DOI:10.1007/BF02349277