Flagellum-mediated adhesion of Trypanosoma congolense to bovine aorta endothelial cells

We studied the interaction between Trypanosoma congolense and bovine aorta endothelial (BAE) cell monolayers. Our findings suggest that trypanosomes adhere predominantly to the flattened, peripheral cell surface domains as well as to filamentous endothelial outgrowths that are present during in vitr...

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Bibliographic Details
Published in:Parasitology research (1987) 1995-05, Vol.81 (5), p.412-420
Main Authors: HEMPHILL, A, ROSS, C. A
Format: Article
Language:English
Subjects:
Adhesiveness
Animals
Aorta
Biological and medical sciences
Cattle
Cell Division
Cells, Cultured
Collagen - pharmacology
Endothelium, Vascular - cytology
Endothelium, Vascular - parasitology
Experimental protozoal diseases and models
Extracellular Matrix Proteins - metabolism
Fibronectins - pharmacology
Flagella - metabolism
Flagella - ultrastructure
Infectious diseases
Laminin - pharmacology
Medical sciences
Microscopy, Electron
Microscopy, Electron, Scanning
Mucins - pharmacology
N-Acetylneuraminic Acid
Parasitic diseases
Protozoal diseases
Sialic Acids - metabolism
Submandibular Gland
Trypanosoma congolense - metabolism
Trypanosoma congolense - ultrastructure
Vitronectin - pharmacology
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Summary:We studied the interaction between Trypanosoma congolense and bovine aorta endothelial (BAE) cell monolayers. Our findings suggest that trypanosomes adhere predominantly to the flattened, peripheral cell surface domains as well as to filamentous endothelial outgrowths that are present during in vitro cultivation in non-confluent monolayers. Adhesion is mediated exclusively by the flagellum in a distinct geometrical order with respect to the flagellar cytoskeleton. Thus, it is possible to define exactly the trypanosomal cell surface domain involved in the attachment process. After 24-48 h of cultivation on monolayers, trypanosomes start to develop short, filopodia-like flagellar protrusions, which serve as additional elements in assisting parasite attachment. Small filaments (3-5 nm) also serve as cross-links between flagellar and endothelial cell surface membranes. Lectin-gold labeling shows that these cross-links contain sialic acid residues. In vitro assays confirm that sialic acid is involved in the adhesion process, whereas the extracellular matrix (ECM) proteins fibronectin, collagen, laminin and vitronectin are not. The presence of T. congolense exhibits a mitogenic effect on BAE cells.
ISSN:0932-0113
1432-1955
DOI:10.1007/BF00931503