Subcutaneous Growth of Staphylococcus aureus Concomitantly Inoculated with Ehrlich Ascites Tumor Cells

• Published in: MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(9), pp.725-728
• Main Authors: Murai, Miyo, Seki, Keiko, Sakurada, Junji, Usui, Akemi, Masuda, Shogo
• Format: Article
• Language: English
• Subjects: Abscess - microbiology; Abscess - pathology; Animals; Bacteriology; Biological and medical sciences; Carcinoma, Ehrlich Tumor - microbiology; Carcinoma, Ehrlich Tumor - pathology; Colony Count, Microbial; Ehrlich ascites tumor cells; Female; Fundamental and applied biological sciences. Psychology; Intratumoral growth; Kidney - microbiology; Mice; Mice, Inbred ICR; Microbiology; Neoplasm Transplantation; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Skin - microbiology; Skin - pathology; Skin Neoplasms - microbiology; Skin Neoplasms - pathology; Staphylococcal Infections - microbiology; Staphylococcal Infections - pathology; Staphylococcus aureus; Staphylococcus aureus - growth & development
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1995.tb03248.x

Intratumoral growth of Staphylococcus aureus Cowan I-derived AP332 was examined by subcutaneous inoculation of cocci in doses ranging from 18 to 1.8×105CFU with Ehrlich ascites tumor cells. Inoculation of 18CFU AP332 resulted in staphylococcal growth in one of five mice, and the proportion of mice established intratumoral infection increased with the initial inocula. Six other strains of S. aureus also grew in the tumor tissue, and none of the three strains of coagulase-negative staphylococci grew at all. Ethanol-killed tumor cells did not promote staphylococcal growth as vigorously as the live tumor cells, especially when the initial inoculum of AP332 was smaller than 104CFU.