Separation of Mono- and Divinyl Chlorophyll Species by High-Performance Liquid Chromatography Using an Octadecyl Polyvinyl Alcohol Polymer Column

Most of the chlorophylls and their related compounds from nonesterified chlorophyllide to esterified pheophytin were separated by high-performance liquid chromatography using a wide pore, C 18 polyvinyl alcohol polymer column with an elution using a binary gradient starting from a buffered mobile ph...

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Bibliographic Details
Published in:Analytical biochemistry 1995-10, Vol.231 (1), p.225-229
Main Authors: Shioi, Y., Watanabe, K., Takamiya, K.I., Garrido, J.L., Zapata, M.
Format: Article
Language:English
Subjects:
Chlorophyll - isolation & purification
Chlorophyllides - isolation & purification
Chromatography, High Pressure Liquid - methods
Pheophytins - isolation & purification
Plants
Polyvinyl Alcohol
Rhodobacter
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Summary:Most of the chlorophylls and their related compounds from nonesterified chlorophyllide to esterified pheophytin were separated by high-performance liquid chromatography using a wide pore, C 18 polyvinyl alcohol polymer column with an elution using a binary gradient starting from a buffered mobile phase. The high selectivity of this system enabled not only the separation of common chlorophyll species but also the resolution of structurally similar compounds such as mono- and divinyl chlorophyllide and pheophorbide species which usually coelute on monomeric bonded phases. The method is successfully applied to analyses of the pigments extracted from green and etiolated tissues of higher plants and photosynthetic bacterial cells.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1995.1524