Opposite actions of transforming growth factor-beta 1 on the gene expression of atrial natriuretic peptide biological and clearance receptors in a murine thymic stromal cell line

The regulation of the gene expression of the atrial natriuretic peptide receptor (ANPR) subtypes, ANPR-A, ANPR-B, and ANPR-C, was investigated in a murine thymic stromal cell line, MRL 104.8a. When MRL 104.8a cells were cultured with transforming growth factor (TGF)-beta1, [125I]ANP binding sites in...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1995-09, Vol.118 (3), p.500-507
Main Authors: Agui, T, Xin, X, Cai, Y, Shim, G, Muramatsu, Y, Yamada, T, Fujiwara, H, Matsumoto, K
Format: Article
Language:English
Subjects:
Animals
Atrial Natriuretic Factor - biosynthesis
Atrial Natriuretic Factor - genetics
Base Sequence
Cells, Cultured
Down-Regulation - drug effects
Gene Expression Regulation - drug effects
Mice
Molecular Sequence Data
Receptors, Atrial Natriuretic Factor - biosynthesis
Receptors, Atrial Natriuretic Factor - classification
Receptors, Atrial Natriuretic Factor - genetics
Stromal Cells - drug effects
Stromal Cells - physiology
Thymus Gland - cytology
Thymus Gland - drug effects
Thymus Gland - physiology
Transforming Growth Factor beta - pharmacology
Transforming Growth Factor beta - physiology
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Summary:The regulation of the gene expression of the atrial natriuretic peptide receptor (ANPR) subtypes, ANPR-A, ANPR-B, and ANPR-C, was investigated in a murine thymic stromal cell line, MRL 104.8a. When MRL 104.8a cells were cultured with transforming growth factor (TGF)-beta1, [125I]ANP binding sites increased with increasing dose of TGF-beta1. These binding sites were identified as ANPR-C by a displacement experiment with ANPR-C-specific ligand, C-ANF, and by the affinity cross-linking of the [125I]ANP binding sites with a chemical cross-linker to determine the molecular weight of the ANPR. This augmentation of the ANPR-C expression was elucidated to occur at the transcriptional level by Northern blot experiment, comparison of the relative amounts of mRNA by reverse transcription (RT)-PCR, and in vitro nuclear transcription assay. Conversely, the expression of the ANP biological receptors, ANPR-A and ANPR-B, was shown to be down-regulated by TGF-beta1. These data suggest that TGF-beta1 regulates the gene expression of ANPRs in the thymic stromal cells and that ANP and TGF-beta1 might affect the thymic stromal cell functions.
ISSN:0021-924X