Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromos...

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Bibliographic Details
Published in:Archives of microbiology 1995-12, Vol.164 (6), p.444-447
Main Authors: FUJITA, M, MOROOKA, T, FUJIMOTO, S, MORIYA, T, AMAKO, K
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Blotting, Southern
Campylobacter fetus - classification
Campylobacter fetus - genetics
Conserved Sequence
DNA Primers - genetics
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Membrane Glycoproteins
Microbiology
Molecular Sequence Data
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Summary:Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF02529743