Effect of a coffee lipid (cafestol) on regulation of lipid metabolism in CaCo-2 cells

The influence of cafestol, a lipid component found in boiled coffee, on low density lipoprotein (LDL) and lipid metabolism was investigated in CaCo-2 cells cultured on filter membranes. The rate of uptake and degradation of 125I-labeled tyramine cellobiose-LDL was increased 50% in CaCo-2 cells incub...

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Bibliographic Details
Published in:Journal of lipid research 1995-10, Vol.36 (10), p.2079-2089
Main Authors: Ranheim, T. (University of Oslo, Norway.), Halvorsen, B, Huggett, A.C, Blomhoff, R, Drevon, C.A
Format: Article
Language:English
Subjects:
Cell Line
Chloramphenicol O-Acetyltransferase - genetics
Cholesterol - biosynthesis
Cholesterol - metabolism
Diterpenes - chemistry
Diterpenes - pharmacology
Humans
Hydroxycholesterols - pharmacology
Intestines - cytology
Intestines - drug effects
Lipid Metabolism
LIPOPROTEINAS
LIPOPROTEINE
Lipoproteins, LDL - metabolism
METABOLISME DES LIPIDES
METABOLISMO DE LIPIDOS
Molecular Structure
Oleic Acid
Oleic Acids - metabolism
Receptors, LDL - analysis
Receptors, LDL - genetics
RNA, Messenger - biosynthesis
Triglycerides - metabolism
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Summary:The influence of cafestol, a lipid component found in boiled coffee, on low density lipoprotein (LDL) and lipid metabolism was investigated in CaCo-2 cells cultured on filter membranes. The rate of uptake and degradation of 125I-labeled tyramine cellobiose-LDL was increased 50% in CaCo-2 cells incubated with cafestol (20 micrograms/ml, 63 micromolar) for 24 h, whereas in cells incubated with 25-hydroxycholesterol (10 micrograms/ml, 25 micromolar) the rate of uptake and degradation showed a 30% decrease. A mixture of kahweol and cafestol, both natural components of coffee beans, modestly enhanced the rate of LDL uptake and degradation, as compared to pure cafestol. Incubation of cafestol with CaCo-2 cells induced a 3-fold up-regulation of LDL receptor mRNA, as compared to control cells. In contrast, incubation of the cells with 25-hydroxycholesterol produced a 30% decrease of LDL receptor expression. CaCo-2 cells were transfected with a promoter region containing the sterol regulatory element-1 (SRE-1) coupled to the reporter gene chloramphenicol acetyltransferase (CAT). When cells transfected with SRE-1 promoter were incubated with cafestol, there was a 20% up-regulation of CAT activity, whereas 25-hydroxycholesterol abolished this activity. Cafestol contributed to a significantly lowered secretion of cholesteryl ester and triacylglycerol, regardless of the radiolabeled precursor used ([2-14C]acetic acid, [1,2,3-3H]glycerol, [3H]water, and [1-14C]oleic acid). This reduction in secretion of lipids was accompanied by an increase in trichloroacetic acid-soluble activity when radiolabeled oleic acid was used as a tracer. We conclude that cafestol promotes an enhanced rate of uptake and degradation of LDL, probably due to an increase in transcription of LDL receptor mRNA and a reduced secretion of cholesteryl ester and triacylglycerol in CaCo-2 cells
ISSN:0022-2275
1539-7262