Assignment of the βB1 Crystallin Gene (CRYBB1) to Human Chromosome 22 and Mouse Chromosome 5

• Published in: Genomics (San Diego, Calif.) Calif.), 1995-10, Vol.29 (3), p.712-718
• Main Authors: HULSEBOS, T.J.M., GILBERT, D.J., DELATTRE, O., SMINK, L.J., DUNHAM, I., WESTERVELD, A., THOMAS, G., JENKINS, N.A., COPELAND, N.G.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cattle; Chromosome Mapping; Chromosomes, Human, Pair 22; Classical genetics, quantitative genetics, hybrids; Cosmids; Crystallins - biosynthesis; Crystallins - genetics; Exons; Fundamental and applied biological sciences. Psychology; Gene Library; Genetic Complementation Test; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Hybrid Cells; Mice - genetics; Molecular Sequence Data; Rats; Sequence Homology, Nucleic Acid; Species Specificity
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1995.9947

By using primers complementary to the rat βB1 crystallin gene sequence, we amplified exons 5 and 6 of the orthologous human gene (CRYBB1). The amplified human segments displayed greater than 88% sequence homology to the corresponding rat and bovine sequences.CRYBB1was assigned to the group 5 region in 22q11.2–q12.1 by hybridizing the exon 6 PCR product to somatic cell hybrids containing defined portions of human chromosome 22. The exon 5 and exon 6 PCR products ofCRYBB1were used to localize, by interspecific backcross mapping, the mouse gene (Crybb1) to the central portion of chromosome 5. Three other β crystallin genes (βB2(−1), βB3, and βA4) have previously been mapped to the same regions in human and mouse. We demonstrate that the βB1 and βA4 crystallin genes are very closely linked in the two species. These assignments complete the mapping and identification of the human and mouse homologues of the major β crystallins genes that are expressed in the bovine lens.