Nitric oxide induces c- fos gene expression via cyclic AMP response element binding protein (CREB) phosphorylation in rat retinal pigment epithelium

We have examined whether nitric oxide (NO) induces the expression of c- fos proto-oncogene and the phosphorylation of cyclic AMP response element binding protein (CREB) in the rat retina. NO donor sodium nitroprusside (SNP) was injected into the vitreal cavity of the eye, and the retina and retinal...

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Bibliographic Details
Published in:Brain research 1995-10, Vol.696 (1), p.140-144
Main Authors: Ohki, Kazutaka, Yoshida, Kazuhiko, Hagiwara, Masatoshi, Harada, Takayuki, Takamura, Mariko, Ohashi, Tsutomu, Matsuda, Hidehiko, Imaki, Junko
Format: Article
Language:English
Subjects:
Activating Transcription Factor 2
Animals
Biological and medical sciences
c- fos
Cyclic AMP response element binding protein
Cyclic AMP Response Element-Binding Protein - metabolism
Eye and associated structures. Visual pathways and centers. Vision
Fundamental and applied biological sciences. Psychology
Gene Expression - drug effects
Genes, fos - drug effects
Immunohistochemistry
In Situ Hybridization
Leucine Zippers
Male
Nitric oxide
Nitric Oxide - pharmacology
Nitroprusside - pharmacology
PC12 Cells
Phosphorylation
Pigment Epithelium of Eye - drug effects
Pigment Epithelium of Eye - metabolism
Rats
Rats, Inbred Strains
Retina - drug effects
Retina - metabolism
Retinal pigment epithelium
RNA Probes
Transcription Factors
Vertebrates: nervous system and sense organs
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Summary:We have examined whether nitric oxide (NO) induces the expression of c- fos proto-oncogene and the phosphorylation of cyclic AMP response element binding protein (CREB) in the rat retina. NO donor sodium nitroprusside (SNP) was injected into the vitreal cavity of the eye, and the retina and retinal pigment epithelium (RPE) was analysed by in situ hybridization using single-stranded RNA probes for c- fos transcripts and by immunocytochemistry using an anti-phospho Ser-133 antibody 20 min after the injection of SNP, phosphorylated-CREB immunoreactivity was found in RPE cells and weakly in some cells of the INL. Forty-five min after the SNP injection, the expression of c- fos mRNA was detected in the RPE. These results suggested that NO induced the c- fos expression via the phosphorylation of CREB in RPE cells, as it had been demonstrated in PC12 cells that the transcription of c- fos gene was activated by the CREB phosphorylation.
ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(95)00914-C