Parvalbumin-immunoreactive proteins in the nervous system of planarians

1. Using immunological methods, we have identified parvalbumin-like material in the triclade flatworms, Polycelis nigra, Polycelis auriculata, Crenobia alpina, Dugesia tahitiensis, and Dugesia polychroa. 2. Western immunoblot analysis of these five species revealed heat stable parvalbumin-immunoreac...

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Bibliographic Details
Published in:Cellular and molecular neurobiology 1995-08, Vol.15 (4), p.401-410
Main Authors: Hutticher, A, Kerschbaum, H H, Kainz, V, Bito, M, Hermann, A
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Calcium-Binding Proteins - analysis
Immunohistochemistry
Molecular Weight
Nervous System - chemistry
Parvalbumins - analysis
Parvalbumins - immunology
Planaria
Planarians - chemistry
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Summary:1. Using immunological methods, we have identified parvalbumin-like material in the triclade flatworms, Polycelis nigra, Polycelis auriculata, Crenobia alpina, Dugesia tahitiensis, and Dugesia polychroa. 2. Western immunoblot analysis of these five species revealed heat stable parvalbumin-immunoreactive proteins between 32 kD and 44 kD. 3. Proteins at 19 and 32 kD revealed intense labeling with 45Ca2+. 4. Double immunodiffusion of planarian supernatants showed complete fusion of precipitates, indicating immunological relatedness of the parvalbumin-like material among the species investigated. 5. Immunocytochemical studies exhibit parvalbumin-immunoreactive material exclusively in neurons supporting the notion of an early evolutionary appearance of these proteins in the nervous system.
ISSN:0272-4340
1573-6830
DOI:10.1007/BF02071876