A Continuous Culture of Pluripotent Fetal Hepatocytes Derived from the 8-Day Epiblast of the Pig

Continuous cultures of pluripotent parenchymal hepatocytes were derived from the epiblasts of 8-day-old pig blastocysts. The cells were polygonal and had phase-contrast dark, granular cytoplasm with prominent nuclei and nucleoli. These feeder-dependent cell cultures differentiated into large, multic...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 1994-12, Vol.30A (12), p.843-850
Main Authors: Neil C. Talbot, Caird E. Rexroad, Jr, Anne M. Powell, Pursel, Vernon G., Thomas J. Caperna, Ogg, Sherry L., Neil D. Nel
Format: Article
Language:English
Subjects:
Albumins
alpha-Fetoproteins - genetics
Animals
Blotting, Northern
Cell culture techniques
Cell Differentiation
Cell Division
Cell lines
Cell Nucleus - ultrastructure
Cells, Cultured
Cellular Models
Culture Media
Culture Media, Conditioned
Cultured cells
Cytoplasm - ultrastructure
Epithelial cells
Feeder cells
Fibrinogen - genetics
Germ cells
Guinea Pigs
Hepatocytes
Immunoblotting
Liver
Liver - embryology
Liver - ultrastructure
Pluripotent stem cells
RNA, Messenger - metabolism
Serum Albumin - genetics
Swine
Transforming Growth Factor beta - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Continuous cultures of pluripotent parenchymal hepatocytes were derived from the epiblasts of 8-day-old pig blastocysts. The cells were polygonal and had phase-contrast dark, granular cytoplasm with prominent nuclei and nucleoli. These feeder-dependent cell cultures differentiated into large, multicellular, secretory, duct-like structures or formed small canaliculi between individual cells. Alternatively, the cells accumulated droplets that stained intensely with Oil Red O, a lipid-specific stain. Alpha-fetoprotein (AFP), albumin, and β-fibrinogen mRNAs were expressed as the cells differentiated in culture. Serum-free medium that was conditioned by the cells contained transferrin, AFP, and albumin. The growth and viability of the cells were inhibited by transforming growth factor β1 (TGFβ1) at concentrations ≥1 ng/ml. The cell cultures grew slowly with doubling times of 2 to 3 d. One of the cultures, pig inner cell mass-19 (PICM-19), was passaged continuously for over 2 yr [>100 population doublings (PD)] and appears to be an infinitely self-renewing cell population. The stem cell characteristics of the epiblast-derived fetal hepatocytes indicate that the cells may be unique for investigations of liver differentiation and organogenesis.
ISSN:1071-2690
1543-706X
DOI:10.1007/BF02639394