An STS-Based Map of the Human Genome

• Published in: Science (American Association for the Advancement of Science) 1995-12, Vol.270 (5244), p.1945-1954
• Main Authors: Hudson, Thomas J., Stein, Lincoln D., Gerety, Sebastian S., Ma, Junli, Castle, Andrew B., Silva, James, Slonim, Donna K., Baptista, Rafael, Kruglyak, Leonid, Xu, Shu-Hua, Hu, Xintong, Angela M. E. Colbert, Rosenberg, Carl, Reeve-Daly, Mary Pat, Rozen, Steve, Hui, Lester, Wu, Xiaoyun, Vestergaard, Christina, Wilson, Kimberly M., Bae, Jane S., Maitra, Shanak, Ganiatsas, Soula, Evans, Cheryl A., DeAngelis, Margaret M., Ingalls, Kimberly A., Nahf, Robert W., Horton, Lloyd T., Anderson, Michele Oskin, Collymore, Alville J., Ye, Wenjuan, Kouyoumjian, Vardouhie, Zemsteva, Irena S., Tam, James, Devine, Richard, Courtney, Dorothy F., Renaud, Michelle Turner, Nguyen, Huy, O'Connor, Tara J., Fizames, Cécile, Fauré, Sabine, Gyapay, Gabor, Dib, Colette, Morissette, Jean, Orlin, James B., Birren, Bruce W., Goodman, Nathan, Weissenbach, Jean, Hawkins, Trevor L., Foote, Simon, Page, David C., Lander, Eric S.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Line; Chromosome Mapping; Chromosomes; Chromosomes, Artificial, Yeast; Classical genetics, quantitative genetics, hybrids; Databases, Factual; Fundamental and applied biological sciences. Psychology; Gene Expression; Genetic loci; Genetic mapping; Genetic Markers; Genetic screening; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genome, Human; Genomes; Genomics; Human; Human genetics; Human genome; Human Genome Project; Humans; Hybrid Cells; Libraries; Polymerase Chain Reaction; Science and Society; Sequence Analysis, DNA; Sequence Tagged Sites
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.270.5244.1945

A physical map has been constructed of the human genome containing 15,086 sequence-tagged sites (STSs), with an average spacing of 199 kilobases. The project involved assembly of a radiation hybrid map of the human genome containing 6193 loci and incorporated a genetic linkage map of the human genome containing 5264 loci. This information was combined with the results of STS-content screening of 10,850 loci against a yeast artificial chromosome library to produce an integrated map, anchored by the radiation hybrid and genetic maps. The map provides radiation hybrid coverage of 99 percent and physical coverage of 94 percent of the human genome. The map also represents an early step in an international project to generate a transcript map of the human genome, with more than 3235 expressed sequences localized. The STSs in the map provide a scaffold for initiating large-scale sequencing of the human genome.