Increase in Osmotolerance of Rhizobium fredii Soybean Isolate BD32 by the proB proA Operon of Escherichia coli

The proB proA operons (which are blocked by the feedback inhibition of proline production) of Escherichia coli wild type or with tile mutation proBosm, blocking feedback inhibition effect of proline production, were cloned in a broad host range shuttle vector pVA 12-2. The hybrid plasmids pLVA(proB+...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-12, Vol.217 (3), p.796-801
Main Authors: Neumivakin, L.V., Solovjev, V.P., Sokhansanj, A., Tilba, V.A., Moseiko, N.A., Shachbasian, R.V., Piruzian, E.S.
Format: Article
Language:English
Subjects:
Aldehyde Oxidoreductases - genetics
Cloning, Molecular
ESCHERICHIA COLI
Escherichia coli - genetics
Genes, Bacterial
Genetic Complementation Test
Glutamate-5-Semialdehyde Dehydrogenase
Glycine max
Operon
Phosphotransferases (Carboxyl Group Acceptor) - genetics
PLASMIDE
PLASMIDIOS
PRESION OSMOTICA
PRESSION OSMOTIQUE
PROLINA
PROLINE
Proline - physiology
RHIZOBIUM
Rhizobium - genetics
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
Water-Electrolyte Balance
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Summary:The proB proA operons (which are blocked by the feedback inhibition of proline production) of Escherichia coli wild type or with tile mutation proBosm, blocking feedback inhibition effect of proline production, were cloned in a broad host range shuttle vector pVA 12-2. The hybrid plasmids pLVA(proB+A+) and pNSA(proBosm proA) were transferred into a low level osmotolerance Rhizobium fredii strain BD32. Both types of transconjugants were characterised by increased osmotolerance in a minimal medium supplied with 0.4-0.8 M NaCl but in the case of pNSA the effect was more significant. The strain BD32/pNSA had all increased level of intracellular proline concentration. Practical application of the increase in Rhizobium resistance to the stress factors is discussed.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2842