Purification and characterization of cytoplasmic dynein of rabbit liver

Cutplasmic dynein is a microtubule-dependent motor protein, which plays a role in intracellular transport. However, there have been a few studies regarding the role of cytoplasmic dynein in the liver. Purification of cytoplasmic dynein from rabbit liver took advantage of the affinity of microtubule-...

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Bibliographic Details
Published in:Journal of hepatology 1995-07, Vol.23 (1), p.66-70
Main Authors: Nakajima, Ko, Komatsu, Masafumi, Toyoshima, Itaru, Kuramitsu, Tomoyuki, Ono, Tsuyoshi, Funaoka, Masato, Kato, Junji, Masamune, Osamu
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cytoplasmic dynein
Dyneins - analysis
Dyneins - isolation & purification
Dyneins - physiology
Electrophoresis, Polyacrylamide Gel
Hepatocyte
Intracellular transport
Liver - chemistry
Liver - cytology
Liver - ultrastructure
Medical sciences
Microscopy, Electron
Microscopy, Interference
Microtubules
Microtubules - chemistry
Microtubules - physiology
Microtubules - ultrastructure
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Neurology
Rabbits
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Summary:Cutplasmic dynein is a microtubule-dependent motor protein, which plays a role in intracellular transport. However, there have been a few studies regarding the role of cytoplasmic dynein in the liver. Purification of cytoplasmic dynein from rabbit liver took advantage of the affinity of microtubule-dependent motor proteins for microtubules. Purified dynein contained heavy chain (450 kDa), intermediate chain (75 kDa), light chains (45–58 kDa) and dynactin (150 kDa). The subunit composition was consistent with previously reported data on brain cytolplasmic dynein. Microtubules prepared from bovine brain were driven by purified cytoplasmic dynein from rabbit liver, and movements of microtubules were visualized by video-enhanced differential interference contrast microscopy. The mean velocity of the motile microtubules was 1.09±0.13 μm/s. Our study provides evidence of rapid intracellular transport in hepatocytes controlled by cytoplasmic dynein.
ISSN:0168-8278
1600-0641
DOI:10.1016/0168-8278(95)80312-2