Mapping of two overlapping linear epitopes in Pfg27 recognized by Plasmodium falciparum transmission-blocking monoclonal antibodies

We have reported previously the production of Plasmodium falciparum transmission-blocking monoclonal antibodies (mAb) recognizing a reduction-insensitive cross-reacting epitope in the gametocyte antigen Pfg27 and the gamete surface antigens Pfs230 and Pfs4845. In this study, the amino acid sequence...

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Bibliographic Details
Published in:Vaccine 1995, Vol.13 (13), p.1161-1169
Main Authors: Ploton, Isabelle N., Wizel, Benjamin, Viscidi, Raphael, Kumar, Nirbhay
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antigen-Antibody Reactions
Antigens, Protozoan - genetics
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Conserved Sequence
Epitope Mapping
Escherichia coli
Female
Fundamental and applied biological sciences. Psychology
Genes, Protozoan
Genetic Code
linear epitope
Malaria, Falciparum - immunology
Malaria, Falciparum - transmission
Mice
Molecular Sequence Data
monoclonal antibodies
Pfg27
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
Plasmodium falciparum - physiology
Protein Conformation
Protozoa
Recombinant Proteins - biosynthesis
transmission-blocking immunity
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Summary:We have reported previously the production of Plasmodium falciparum transmission-blocking monoclonal antibodies (mAb) recognizing a reduction-insensitive cross-reacting epitope in the gametocyte antigen Pfg27 and the gamete surface antigens Pfs230 and Pfs4845. In this study, the amino acid sequence of this epitope in Pfg27 was determined. First, the epitope was localized near the N terminus of the protein by probing recombinant overlapping fragments spanning Pfg27 with transmission-blocking mAb in immunoblot experiments. The amino acid sequence of the epitope was then determined by using overlapping synthetic peptides spanning the smallest immunoreactive recombinant fragment in an ELISA. The sequence KPLDKFGNIYDYHYEH (amino acids 10–25 in the Pfg27 sequence) was shown to contain two overlapping epitopes recognized by transmission-blocking mAb. Comparison of the sequence of the gene encoding Pfg27 in seven different P. falciparum strains demonstrated that these sequential epitopes are totally conserved. Immunization of mice with synthetic peptides derived from Pfg27, conjugated with keyhole limpet hemocyanin (KLH) and formulated in Freund's adjuvant or alum, resulted in the production of antibodies capable of recognizing the peptides as well as the native Pfg27.
ISSN:0264-410X
1873-2518
DOI:10.1016/0264-410X(95)00033-W