Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces. GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei β-tubulin promo...

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Bibliographic Details
Published in:Current genetics 1995-12, Vol.29 (1), p.100-102
Main Authors: Christiansen, S K, Knudsen, S, Giese, H
Format: Article
Language:English
Subjects:
Ascomycota - genetics
Aspergillus nidulans - genetics
Erysiphe graminis
Gene Expression Regulation
Genetic Engineering - methods
Glucuronidase - biosynthesis
Glucuronidase - genetics
Hordeum - microbiology
Plants - microbiology
Promoter Regions, Genetic
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Time Factors
Transformation, Genetic
Tubulin - genetics
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Summary:Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces. GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei β-tubulin promoter. Three heterologous promoters, onefrom Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00313200