Capsular polysaccharide serotypes of coagulase-positive staphylococci associated with tenosynovitis, osteomyelitis, and other invasive infections in chickens and turkeys: evidence for new capsular types

To study the role of capsular polysaccharides in staphylococcal infections in chickens and turkeys, we surveyed 101 coagulase-positive isolates obtained from investigators in geographically diverse locales in the United States and Canada. Fifty-six isolates were obtained from chickens; 51 (91%) were...

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Bibliographic Details
Published in:Avian diseases 1994-10, Vol.38 (4), p.762-771
Main Authors: Daum, R.S. (University of Chicago, Chicago, IL.), Fattom, A, Freese, S, Karakawa, W
Format: Article
Language:English
Subjects:
AMINOAZUCARES
AMINOSUCRE
Animals
Antibodies
Antiserum
Bacterial Typing Techniques - veterinary
BACTERIOSE
BACTERIOSIS
Chemical suspensions
Chickens
Chickens - microbiology
DINDON
ENCAPSULACION
ENCAPSULATION
ENCUESTAS
ENQUETE
IMMUNOLOGIE
Infections
INMUNOLOGIA
PAVO
Phagocytosis
PODER PATOGENO
POLISACARIDOS
POLLO
POLYHOLOSIDE
Polysaccharides
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - isolation & purification
POULET
Poultry Diseases - microbiology
POUVOIR PATHOGENE
SEROTIPOS
SEROTYPE
Sodium
Staphylococcal Infections - microbiology
Staphylococcal Infections - veterinary
STAPHYLOCOCCUS
Staphylococcus - classification
STAPHYLOCOCCUS AUREUS
TECHNIQUE ANALYTIQUE
TECHNIQUE IMMUNOLOGIQUE
TECNICAS ANALITICAS
TECNICAS INMUNOLOGICAS
Turkeys
Turkeys - microbiology
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Summary:To study the role of capsular polysaccharides in staphylococcal infections in chickens and turkeys, we surveyed 101 coagulase-positive isolates obtained from investigators in geographically diverse locales in the United States and Canada. Fifty-six isolates were obtained from chickens; 51 (91%) were type 5, and 5 (9%) were type 8. Forty isolates were obtained from turkeys; 13 (33%) were type 5, 15 (38%) were type 8, and 12 (29%) were untypable. Five type 5 isolates were obtained from poultry of uncertain species. Two untypable turkey isolates were studied further. Rabbit serum obtained after immunization with formalin killed staphylococci was type-specific as assessed by a tube agglutination test and promoted opsonophagocytosis of the isolate that had been used for immunization. Chromatography of carboxy-reduced capsular polysaccharide from one of the isolates suggested that mannoseaminouronic acid was present in the unreduced polysaccharide. The spectrum obtained by 13C-nuclear magnetic resonance suggested the presence of N-acetyl sugars such as mannoseaminouronic acid and 6-deoxy sugars such as N-acetyl fucosamine. The structure of the polysaccharide differed from that of the type 5 and type 8 capsular polysaccharides. Thus, poultry isolates can be classified into at least four serotypes on the basis of their capsular polysaccharides. This observation provides a new focus for investigation into the epidemiology and virulence determinants of this important pathogen in chickens and turkeys
ISSN:0005-2086
1938-4351
DOI:10.2307/1592112