Cloning and characterisation of the rabbit 5‐HT1Dα and 5‐HT1Dβ receptors

The genes encoding the rabbit 5HT1Dα and 5HT1Dβ receptors have been cloned. The deduced amino acid sequence of these receptors shows 91–92% amino acid sequence identity with their human homologues, and similar high sequence identity with homologues from other species. The receptors were transiently...

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Bibliographic Details
Published in:FEBS letters 1995-12, Vol.377 (1), p.73-76
Main Authors: Harwood, Gail, Lockyer, Michael, Giles, Heather, Fairweather, Neil
Format: Article
Language:English
Subjects:
5-Hydroxytryptamine
Amino Acid Sequence
Animals
Binding, Competitive
Cell Line
Cloning, Molecular
G-protein coupled receptor
Humans
Ketanserin - metabolism
Molecular Sequence Data
Rabbit
Rabbits
Receptor, Serotonin, 5-HT1B
Receptor, Serotonin, 5-HT1D
Receptors, Serotonin - chemistry
Receptors, Serotonin - genetics
Receptors, Serotonin - metabolism
Recombinant Proteins
Sequence Homology
Serotonin
Transfection
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Description
Summary:The genes encoding the rabbit 5HT1Dα and 5HT1Dβ receptors have been cloned. The deduced amino acid sequence of these receptors shows 91–92% amino acid sequence identity with their human homologues, and similar high sequence identity with homologues from other species. The receptors were transiently expressed in COS‐7 cells and exhibit a pharmacological profile closely resembling their human homologues, including a higher affinity of ketanserin for the 5‐HT1Dα subtype. However, sumatriptan had a lower affinity for both the rabbit receptors compared to their human counterparts. This may be accounted for by differences between the primary amino acid sequences of these species homologues.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01308-3