Influence of bile on beta-galactosidase activity of component species of yogurt starter cultures

The influence of bile on beta-galactosidase activity and the cellular integrity of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus were tested. Two strains (OS 1 and OS 2) of L. delbrueckii ssp. bulgaricus and three strains (HC 15, 17, and 18) of S. salivariu...

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Bibliographic Details
Published in:Journal of dairy science 1994-12, Vol.77 (12), p.3532-3537
Main Authors: Noh, D.O. (Oklahoma State University, Stillwater), Gilliland, S.E
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
BETA GALACTOSIDASA
BETA GALACTOSIDASE
beta-Galactosidase - metabolism
BILE
Bile - physiology
BILIS
Biological and medical sciences
Cell Membrane Permeability
CULTIVOS INICIADORES
CULTURE STARTER
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
Food industries
Fundamental and applied biological sciences. Psychology
INHIBIDORES DE ENZIMAS
INHIBITEUR D'ENZYME
Lactobacillus - enzymology
LACTOBACILLUS DELBRUECKII
LAIT FERMENTE
LECHE FERMENTADA
Milk and cheese industries. Ice creams
PERMEABILIDAD
PERMEABILITE
STREPTOCOCCUS
Streptococcus - enzymology
Yogurt - microbiology
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Summary:The influence of bile on beta-galactosidase activity and the cellular integrity of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus were tested. Two strains (OS 1 and OS 2) of L. delbrueckii ssp. bulgaricus and three strains (HC 15, 17, and 18) of S. salivarius ssp. thermophilus were studied. In the presence of 0.15% oxgall, beta-galactosidase activity of whole cells of both species was significantly increased. However, at a higher concentration (.3% oxgall), the beta-galactosidase activity was significantly less than in the presence of 0.15% oxgall. The presence of oxgall did not promote the cell lysis of any of the strains of either species. Additionally, the presence of oxgall did not cause the leakage of the enzyme from the cells. Thus, the presence of oxgall increased the cellular permeability to allow more substrate to enter the cells, thereby increasing beta-galactosidase activity, and, at higher concentrations, was inhibitory to beta-galactosidase activity of both species
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(94)77295-7