Acquisition of viable-like surface properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution

By using centrifugal countercurrent distribution in dextran/polyethylene glycol two-phase system, we show that the acquisition of adsorbed seminal plasma proteins by the sperm cell surface modifies the partition behaviour of bull spermatozoa reproducing that of alive cells. Thus, addition of increas...

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Bibliographic Details
Published in:Biology of the cell 1994, Vol.82 (1), p.75-78
Main Authors: Pascual, ML, Muiño-Blanco, T, Cebrián-Pérez, JA, López-Pérez, MJ
Format: Article
Language:English
Subjects:
Adsorption
Animals
Biological and medical sciences
Cattle
Cell coat. Cell surface
Cell Separation - methods
Cell structures and functions
Cell Survival
Centrifugation - methods
Countercurrent Distribution - methods
Dextrans
Fundamental and applied biological sciences. Psychology
Male
Membrane Proteins - pharmacokinetics
Molecular and cellular biology
partitioning
Polyethylene Glycols
Semen - metabolism
spermatozoa
Spermatozoa - metabolism
viability
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Summary:By using centrifugal countercurrent distribution in dextran/polyethylene glycol two-phase system, we show that the acquisition of adsorbed seminal plasma proteins by the sperm cell surface modifies the partition behaviour of bull spermatozoa reproducing that of alive cells. Thus, addition of increasing concentrations of proteins from the seminal plasma promoted a saturable enhanced affinity of the cells to the polyethylene glycol-rich phase in which alive cells preferentially partition. On the contrary, treatment with Tween provoked the opposite effect.
ISSN:0248-4900
1768-322X
DOI:10.1016/0248-4900(94)90069-8