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Acquisition of viable-like surface properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution

By using centrifugal countercurrent distribution in dextran/polyethylene glycol two-phase system, we show that the acquisition of adsorbed seminal plasma proteins by the sperm cell surface modifies the partition behaviour of bull spermatozoa reproducing that of alive cells. Thus, addition of increas...

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Published in:	Biology of the cell 1994, Vol.82 (1), p.75-78
Main Authors:	Pascual, ML, Muiño-Blanco, T, Cebrián-Pérez, JA, López-Pérez, MJ
Format:	Article
Language:	English
Subjects:	Adsorption Animals Biological and medical sciences Cattle Cell coat. Cell surface Cell Separation - methods Cell structures and functions Cell Survival Centrifugation - methods Countercurrent Distribution - methods Dextrans Fundamental and applied biological sciences. Psychology Male Membrane Proteins - pharmacokinetics Molecular and cellular biology partitioning Polyethylene Glycols Semen - metabolism spermatozoa Spermatozoa - metabolism viability
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cited_by	cdi_FETCH-LOGICAL-c4742-2efe7a97642d75ab7e0ca15f4d139271868271bf00e236914f9dbfa3c7f054243
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container_issue	1
container_start_page	75
container_title	Biology of the cell
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creator	Pascual, ML Muiño-Blanco, T Cebrián-Pérez, JA López-Pérez, MJ
description	By using centrifugal countercurrent distribution in dextran/polyethylene glycol two-phase system, we show that the acquisition of adsorbed seminal plasma proteins by the sperm cell surface modifies the partition behaviour of bull spermatozoa reproducing that of alive cells. Thus, addition of increasing concentrations of proteins from the seminal plasma promoted a saturable enhanced affinity of the cells to the polyethylene glycol-rich phase in which alive cells preferentially partition. On the contrary, treatment with Tween provoked the opposite effect.
doi_str_mv	10.1016/0248-4900(94)90069-8
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Psychology ; Male ; Membrane Proteins - pharmacokinetics ; Molecular and cellular biology ; partitioning ; Polyethylene Glycols ; Semen - metabolism ; spermatozoa ; Spermatozoa - metabolism ; viability</subject><ispartof>Biology of the cell, 1994, Vol.82 (1), p.75-78</ispartof><rights>1994</rights><rights>1994 Société Française des Microscopies and Société Biologie Cellulaire de France</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4742-2efe7a97642d75ab7e0ca15f4d139271868271bf00e236914f9dbfa3c7f054243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0248490094900698$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4024,27923,27924,27925,45780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3481694$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7537569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pascual, ML</creatorcontrib><creatorcontrib>Muiño-Blanco, T</creatorcontrib><creatorcontrib>Cebrián-Pérez, JA</creatorcontrib><creatorcontrib>López-Pérez, MJ</creatorcontrib><title>Acquisition of viable-like surface properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution</title><title>Biology of the cell</title><addtitle>Biol Cell</addtitle><description>By using centrifugal countercurrent distribution in dextran/polyethylene glycol two-phase system, we show that the acquisition of adsorbed seminal plasma proteins by the sperm cell surface modifies the partition behaviour of bull spermatozoa reproducing that of alive cells. Thus, addition of increasing concentrations of proteins from the seminal plasma promoted a saturable enhanced affinity of the cells to the polyethylene glycol-rich phase in which alive cells preferentially partition. On the contrary, treatment with Tween provoked the opposite effect.</description><subject>Adsorption</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell coat. Cell surface</subject><subject>Cell Separation - methods</subject><subject>Cell structures and functions</subject><subject>Cell Survival</subject><subject>Centrifugation - methods</subject><subject>Countercurrent Distribution - methods</subject><subject>Dextrans</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Membrane Proteins - pharmacokinetics</subject><subject>Molecular and cellular biology</subject><subject>partitioning</subject><subject>Polyethylene Glycols</subject><subject>Semen - metabolism</subject><subject>spermatozoa</subject><subject>Spermatozoa - metabolism</subject><subject>viability</subject><issn>0248-4900</issn><issn>1768-322X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNqNkc9u1DAQxiMEKkvhDUDKASE4BGzHseNLpbKCglRRKhXRm-U4Y2Tq_Kkn2XYfhbfFYZc9Ii4ey_P7xjPfZNlzSt5SQsU7wnhdcEXIa8XfpCBUUT_IVlSKuigZu36YrQ7I4-wJ4k9CCFd1dZQdyaqUlVCr7NepvZ09-skPfT64fONNE6AI_gZynKMzFvIxDiPEyQMuBKZ7l1sIAfNmm5sWhzj-lSN0vjchH4PBzizKCXyPeYQNmADtorDQT9G7-Ufi7DD3E0Q7x5he89ZjSjXzUu5p9siZgPBsH4-zbx8_XK0_FecXZ5_Xp-eF5ZKzgoEDaZQUnLWyMo0EYg2tHG9pqZiktajT2ThCgJVCUe5U2zhTWulIxRkvj7NXu7qp2dsZcNKdx2U808Mwo5ayJqQSVQL5DrRxQIzg9Bh9Z-JWU6KXjejFbr3YrRXXfzai6yR7sa8_Nx20B9F-BSn_cp83aE1w0fTW4wEreU2FWtpUO-zOB9j-19f6_cWaspIlbbHTJnvh_qA18UYLmbrQ37-caVleXl_Jy69aJP5kx0PyfeMharQeegutj2An3Q7-3zP_Br6Pyys</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Pascual, ML</creator><creator>Muiño-Blanco, T</creator><creator>Cebrián-Pérez, JA</creator><creator>López-Pérez, MJ</creator><general>Elsevier SAS</general><general>Blackwell Publishing Ltd</general><general>Portland</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Acquisition of viable-like surface properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution</title><author>Pascual, ML ; Muiño-Blanco, T ; Cebrián-Pérez, JA ; López-Pérez, MJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4742-2efe7a97642d75ab7e0ca15f4d139271868271bf00e236914f9dbfa3c7f054243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adsorption</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell coat. Cell surface</topic><topic>Cell Separation - methods</topic><topic>Cell structures and functions</topic><topic>Cell Survival</topic><topic>Centrifugation - methods</topic><topic>Countercurrent Distribution - methods</topic><topic>Dextrans</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Membrane Proteins - pharmacokinetics</topic><topic>Molecular and cellular biology</topic><topic>partitioning</topic><topic>Polyethylene Glycols</topic><topic>Semen - metabolism</topic><topic>spermatozoa</topic><topic>Spermatozoa - metabolism</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pascual, ML</creatorcontrib><creatorcontrib>Muiño-Blanco, T</creatorcontrib><creatorcontrib>Cebrián-Pérez, JA</creatorcontrib><creatorcontrib>López-Pérez, MJ</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pascual, ML</au><au>Muiño-Blanco, T</au><au>Cebrián-Pérez, JA</au><au>López-Pérez, MJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acquisition of viable-like surface properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution</atitle><jtitle>Biology of the cell</jtitle><addtitle>Biol Cell</addtitle><date>1994</date><risdate>1994</risdate><volume>82</volume><issue>1</issue><spage>75</spage><epage>78</epage><pages>75-78</pages><issn>0248-4900</issn><eissn>1768-322X</eissn><coden>BCELDF</coden><abstract>By using centrifugal countercurrent distribution in dextran/polyethylene glycol two-phase system, we show that the acquisition of adsorbed seminal plasma proteins by the sperm cell surface modifies the partition behaviour of bull spermatozoa reproducing that of alive cells. 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source	ScienceDirect Additional Titles
subjects	Adsorption Animals Biological and medical sciences Cattle Cell coat. Cell surface Cell Separation - methods Cell structures and functions Cell Survival Centrifugation - methods Countercurrent Distribution - methods Dextrans Fundamental and applied biological sciences. Psychology Male Membrane Proteins - pharmacokinetics Molecular and cellular biology partitioning Polyethylene Glycols Semen - metabolism spermatozoa Spermatozoa - metabolism viability
title	Acquisition of viable-like surface properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution
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