Silver Staining for Elucidation of the Synlophe in Trichostrongyle Nematodes

Staining techniques are relatively rare in the study of parasitic nematodes. A novel silver-staining method is described for elucidation of the synlophe (a system of longitudinal cuticular ridges), a character of great systematic importance among the trichostrongyloid nematodes. Ridges are stained o...

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Bibliographic Details
Published in:The Journal of parasitology 1995-12, Vol.81 (6), p.1016-1018
Main Authors: Khrustalev, Alexander V., Hoberg, Eric P.
Format: Article
Language:English
Subjects:
Animals
Microscopy
Nematodes
Parasitology
Reagents
Research Notes
Silver
Silver staining
Silver Staining - methods
Terminator regions
Trichostrongyloidea - anatomy & histology
Trichostrongyloidea - classification
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Description
Summary:Staining techniques are relatively rare in the study of parasitic nematodes. A novel silver-staining method is described for elucidation of the synlophe (a system of longitudinal cuticular ridges), a character of great systematic importance among the trichostrongyloid nematodes. Ridges are stained optically black and appear in great contrast to the body of the nematode. This method augments current use of interference contrast for examination of the synlophe. Detailed studies of the configuration of the synlophe in entire specimens are possible with standard light microscopy for the first time.
ISSN:0022-3395
1937-2345
DOI:10.2307/3284062