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Typing of sequential bacterial isolates by pulsed-field gel electrophoresis
We typed 39 sets of multiple bacterial isolates of the same species from patients by pulsed-field gel electrophoresis of genomic DNA (PFGE). Isolates were cultured from different sites or over a 2-week or longer interval. Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Escheric...
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| Published in: | Diagnostic microbiology and infectious disease 1995-08, Vol.22 (4), p.309-314 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c386t-4a9817438c14c83ad9a5f9ed43eb0c8c33afed3d32f3165e83572fd973f158a73 |
| container_end_page | 314 |
| container_issue | 4 |
| container_start_page | 309 |
| container_title | Diagnostic microbiology and infectious disease |
| container_volume | 22 |
| creator | Hartstein, Alan I. Chetchotisakd, Ploenchan Phelps, Charles L. LeMonte, Ann M. |
| description | We typed 39 sets of multiple bacterial isolates of the same species from patients by pulsed-field gel electrophoresis of genomic DNA (PFGE). Isolates were cultured from different sites or over a 2-week or longer interval.
Staphylococcus aureus,
Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli,
Klebsiella pneumoniae, and
Enterobacter cloacae were tested. Excluding
E. cloacae, 28 of 32 sets of isolates (87%) demonstrated only identical or highly related PFGE types. Four of the seven sets of
E. cloacae showed different types. For species other than
E. cloacae, our results suggest that patients are usually colonized and infected with a single strain of these bacterial pathogens. Unlike all of the other tested species,
E. cloacae PFGE typing differences suggested the presence of multiple strains causing colonization and infection. |
| doi_str_mv | 10.1016/0732-8893(95)00139-8 |
| format | article |
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Staphylococcus aureus,
Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli,
Klebsiella pneumoniae, and
Enterobacter cloacae were tested. Excluding
E. cloacae, 28 of 32 sets of isolates (87%) demonstrated only identical or highly related PFGE types. Four of the seven sets of
E. cloacae showed different types. For species other than
E. cloacae, our results suggest that patients are usually colonized and infected with a single strain of these bacterial pathogens. Unlike all of the other tested species,
E. cloacae PFGE typing differences suggested the presence of multiple strains causing colonization and infection.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/0732-8893(95)00139-8</identifier><identifier>PMID: 8582134</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Bacteria - classification ; Bacteria - isolation & purification ; Bacterial Typing Techniques ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; DNA, Bacterial - isolation & purification ; Electrophoresis, Gel, Pulsed-Field ; Fundamental and applied biological sciences. Psychology ; Genome, Bacterial ; Microbiology</subject><ispartof>Diagnostic microbiology and infectious disease, 1995-08, Vol.22 (4), p.309-314</ispartof><rights>1995</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4a9817438c14c83ad9a5f9ed43eb0c8c33afed3d32f3165e83572fd973f158a73</citedby><cites>FETCH-LOGICAL-c386t-4a9817438c14c83ad9a5f9ed43eb0c8c33afed3d32f3165e83572fd973f158a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2911771$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8582134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hartstein, Alan I.</creatorcontrib><creatorcontrib>Chetchotisakd, Ploenchan</creatorcontrib><creatorcontrib>Phelps, Charles L.</creatorcontrib><creatorcontrib>LeMonte, Ann M.</creatorcontrib><title>Typing of sequential bacterial isolates by pulsed-field gel electrophoresis</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>We typed 39 sets of multiple bacterial isolates of the same species from patients by pulsed-field gel electrophoresis of genomic DNA (PFGE). Isolates were cultured from different sites or over a 2-week or longer interval.
Staphylococcus aureus,
Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli,
Klebsiella pneumoniae, and
Enterobacter cloacae were tested. Excluding
E. cloacae, 28 of 32 sets of isolates (87%) demonstrated only identical or highly related PFGE types. Four of the seven sets of
E. cloacae showed different types. For species other than
E. cloacae, our results suggest that patients are usually colonized and infected with a single strain of these bacterial pathogens. Unlike all of the other tested species,
E. cloacae PFGE typing differences suggested the presence of multiple strains causing colonization and infection.</description><subject>Bacteria - classification</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Bacterial</subject><subject>Microbiology</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwmmx2m-QiSPELC17qOWSTSY2ku2uyFfrvTe3So6cZeJ8ZXh6Ezgm-JZhM7zCjRc65oNeiusGYUJHzAzQmnIkcY4YP0XiPHKOTGL8SVIgSj9CIV7wgtByjt8Wmc80ya20W4XsNTe-Uz2qlewjbzcXWqx5iVm-ybu0jmNw68CZbgs_Ag-5D2322AaKLp-jIqoScDXOCPp4eF7OXfP7-_Dp7mOea8mmfl0pwwkrKNSk1p8oIVVkBpqRQY801pcqCoYYWlpJpBZxWrLBGMGpJxRWjE3S1-9uFNlWOvVy5qMF71UC7jpIxjllJigSWO1CHNsYAVnbBrVTYSILl1qHcCpJbQVJU8s-h5OnsYvi_rldg9keDtJRfDrmKWnkbVKNd3GOFIIQxkrD7HQbJxY-DIKN20GgwLiRv0rTu_x6_79SNlA</recordid><startdate>19950801</startdate><enddate>19950801</enddate><creator>Hartstein, Alan I.</creator><creator>Chetchotisakd, Ploenchan</creator><creator>Phelps, Charles L.</creator><creator>LeMonte, Ann M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950801</creationdate><title>Typing of sequential bacterial isolates by pulsed-field gel electrophoresis</title><author>Hartstein, Alan I. ; Chetchotisakd, Ploenchan ; Phelps, Charles L. ; LeMonte, Ann M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-4a9817438c14c83ad9a5f9ed43eb0c8c33afed3d32f3165e83572fd973f158a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Bacteria - classification</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Bacterial</topic><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hartstein, Alan I.</creatorcontrib><creatorcontrib>Chetchotisakd, Ploenchan</creatorcontrib><creatorcontrib>Phelps, Charles L.</creatorcontrib><creatorcontrib>LeMonte, Ann M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hartstein, Alan I.</au><au>Chetchotisakd, Ploenchan</au><au>Phelps, Charles L.</au><au>LeMonte, Ann M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Typing of sequential bacterial isolates by pulsed-field gel electrophoresis</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1995-08-01</date><risdate>1995</risdate><volume>22</volume><issue>4</issue><spage>309</spage><epage>314</epage><pages>309-314</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>We typed 39 sets of multiple bacterial isolates of the same species from patients by pulsed-field gel electrophoresis of genomic DNA (PFGE). Isolates were cultured from different sites or over a 2-week or longer interval.
Staphylococcus aureus,
Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli,
Klebsiella pneumoniae, and
Enterobacter cloacae were tested. Excluding
E. cloacae, 28 of 32 sets of isolates (87%) demonstrated only identical or highly related PFGE types. Four of the seven sets of
E. cloacae showed different types. For species other than
E. cloacae, our results suggest that patients are usually colonized and infected with a single strain of these bacterial pathogens. Unlike all of the other tested species,
E. cloacae PFGE typing differences suggested the presence of multiple strains causing colonization and infection.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8582134</pmid><doi>10.1016/0732-8893(95)00139-8</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0732-8893 |
| ispartof | Diagnostic microbiology and infectious disease, 1995-08, Vol.22 (4), p.309-314 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | Elsevier |
| subjects | Bacteria - classification Bacteria - isolation & purification Bacterial Typing Techniques Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences DNA, Bacterial - isolation & purification Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Genome, Bacterial Microbiology |
| title | Typing of sequential bacterial isolates by pulsed-field gel electrophoresis |
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